Curated Information
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Curated Information Page
PubMed Id: 9315635 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Knudsen ES, Wang JY (1997) Dual mechanisms for the inhibition of E2F binding to RB by cyclin-dependent kinase-mediated RB phosphorylation. Mol Cell Biol 17, 5771-83 9315635
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S608-p - Rb (human)
Orthologous residues
Rb (human): S608‑p, Rb (mouse): S601‑p, Rb (rat): S600‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, electrophoretic mobility shift, mutation of modification site
 Disease tissue studied:  cervical cancer
 Relevant cell lines - cell types - tissues:  C33-A (cervical), HFFF-2 (fibroblast), Rat1 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human, rat
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
E2F1 (human) Disrupts functional assay, co-immunoprecipitation

S612-p - Rb (human)
Orthologous residues
Rb (human): S612‑p, Rb (mouse): S605‑p, Rb (rat): S604‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, electrophoretic mobility shift, mutation of modification site
 Disease tissue studied:  cervical cancer
 Relevant cell lines - cell types - tissues:  C33-A (cervical), HFFF-2 (fibroblast), Rat1 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human, rat
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
E2F1 (human) Disrupts functional assay, co-immunoprecipitation

S780-p - Rb (human)
Orthologous residues
Rb (human): S780‑p, Rb (mouse): S773‑p, Rb (rat): S772‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, electrophoretic mobility shift, mutation of modification site
 Disease tissue studied:  cervical cancer
 Relevant cell lines - cell types - tissues:  C33-A (cervical), HFFF-2 (fibroblast), Rat1 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human, rat
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Effect of modification (process):  cell growth, altered
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
E2F1 (human) Disrupts functional assay, co-immunoprecipitation

S788-p - Rb (human)
Orthologous residues
Rb (human): S788‑p, Rb (mouse): S781‑p, Rb (rat): S780‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, electrophoretic mobility shift, mutation of modification site
 Disease tissue studied:  cervical cancer
 Relevant cell lines - cell types - tissues:  C33-A (cervical), HFFF-2 (fibroblast), Rat1 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human, rat
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Effect of modification (process):  cell growth, altered
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
E2F1 (human) Disrupts functional assay, co-immunoprecipitation

S795-p - Rb (human)
Orthologous residues
Rb (human): S795‑p, Rb (mouse): S788‑p, Rb (rat): S787‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, electrophoretic mobility shift, mutation of modification site
 Disease tissue studied:  cervical cancer
 Relevant cell lines - cell types - tissues:  C33-A (cervical), HFFF-2 (fibroblast), Rat1 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human, rat
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Effect of modification (process):  cell growth, altered
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
E2F1 (human) Disrupts functional assay, co-immunoprecipitation

S807-p - Rb (human)
Orthologous residues
Rb (human): S807‑p, Rb (mouse): S800‑p, Rb (rat): S799‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, electrophoretic mobility shift, mutation of modification site
 Disease tissue studied:  cervical cancer
 Relevant cell lines - cell types - tissues:  C33-A (cervical), HFFF-2 (fibroblast), Rat1 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human, rat
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Effect of modification (process):  cell growth, altered
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
E2F1 (human) Disrupts functional assay, co-immunoprecipitation

S811-p - Rb (human)
Orthologous residues
Rb (human): S811‑p, Rb (mouse): S804‑p, Rb (rat): S803‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, electrophoretic mobility shift, mutation of modification site
 Disease tissue studied:  cervical cancer
 Relevant cell lines - cell types - tissues:  C33-A (cervical), HFFF-2 (fibroblast), Rat1 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human, rat
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Effect of modification (process):  cell growth, altered
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
E2F1 (human) Disrupts functional assay, co-immunoprecipitation

T821-p - Rb (human)
Orthologous residues
Rb (human): T821‑p, Rb (mouse): T814‑p, Rb (rat): T813‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, electrophoretic mobility shift, mutation of modification site
 Disease tissue studied:  cervical cancer
 Relevant cell lines - cell types - tissues:  C33-A (cervical), HFFF-2 (fibroblast), Rat1 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human, rat
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Effect of modification (process):  cell growth, altered
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
E2F1 (human) Disrupts functional assay, co-immunoprecipitation

T826-p - Rb (human)
Orthologous residues
Rb (human): T826‑p, Rb (mouse): T819‑p, Rb (rat): T818‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, electrophoretic mobility shift, mutation of modification site
 Disease tissue studied:  cervical cancer
 Relevant cell lines - cell types - tissues:  C33-A (cervical), HFFF-2 (fibroblast), Rat1 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human, rat
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Effect of modification (process):  cell growth, altered
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
E2F1 (human) Disrupts functional assay, co-immunoprecipitation


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