|
Orthologous residues
|
|
PKN1 (human): T774‑p, PKN1 (mouse): T778‑p, PKN1 (rat): T778‑p
|
|
Characterization
|
|
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling, immunoprecipitation, mutation of modification site, phosphoamino acid analysis, phosphopeptide mapping
|
|
Relevant cell lines - cell types - tissues:
CHO (fibroblast) [EphB1 (human), transfection]
|
|
Cellular systems studied:
cell lines
|
|
Species studied:
hamster
|
|
Enzymes shown to modify site in vitro:
|
|
|
|
Upstream Regulation
|
|
Potential in vivo enzymes for site:
|
|
Type
|
Enzyme
|
Evidence
|
Notes
|
|
KINASE
|
PDK1 (human)
|
transfection of wild-type enzyme, transfection of inactive enzyme
|
|
|
|
Treatments, proteins and their effect on site modification:
|
|
Treatments
|
Referenced Treatments
|
Manipulated Protein
|
Referenced Protein
|
Effect
|
Notes
|
|
insulin
|
|
|
|
increase
|
|
|
wortmannin
|
insulin
|
|
|
no effect upon treatment-induced increase
|
|
|
LY294002
|
insulin
|
|
|
no effect upon treatment-induced increase
|
|
|
|
Downstream Regulation
|
|
Effect of modification (function):
enzymatic activity, induced, molecular association, regulation
|
|
Effect of modification (process):
cytoskeletal reorganization
|
|
Modification regulates interactions with:
|
|
Interacting molecule
|
Interacting domains
|
Effect
|
Consequences (function)
|
Consequences (process)
|
Detection assays
|
|
PDK1 (human)
|
|
Induces
|
|
|
co-immunoprecipitation, electrophoretic visualization
|
|
