Curated Information
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Curated Information Page
PubMed Id: 10792047 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Dong LQ, et al. (2000) Phosphorylation of protein kinase N by phosphoinositide-dependent protein kinase-1 mediates insulin signals to the actin cytoskeleton. Proc Natl Acad Sci U S A 97, 5089-94 10792047
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S773-p - PKN1 (human)
Orthologous residues
PKN1 (human): S773‑p, PKN1 (mouse): S777‑p, PKN1 (rat): S777‑p
Characterization
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE PDK1 (human)

T774-p - PKN1 (human)
Orthologous residues
PKN1 (human): T774‑p, PKN1 (mouse): T778‑p, PKN1 (rat): T778‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, immunoprecipitation, mutation of modification site, phosphoamino acid analysis, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  CHO (fibroblast) [EphB1 (human), transfection]
 Cellular systems studied:  cell lines
 Species studied:  hamster
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE PDK1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PDK1 (human) transfection of inactive enzyme, transfection of wild-type enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
wortmannin insulin no effect upon treatment-induced increase
LY294002 insulin no effect upon treatment-induced increase
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced, molecular association, regulation
 Effect of modification (process):  cytoskeletal reorganization
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
PDK1 (human) Induces electrophoretic visualization, co-immunoprecipitation


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