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Orthologous residues
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TOPORS (human): S718‑p, TOPORS (mouse): S718‑p
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Characterization
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Methods used to characterize site in vivo:
[32P] bio-synthetic labeling, immunoprecipitation, mutation of modification site, western blotting
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Disease tissue studied:
bone cancer
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Relevant cell lines - cell types - tissues:
293T (epithelial), U2OS (bone cell)
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Cellular systems studied:
cell lines
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Species studied:
human
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Enzymes shown to modify site in vitro:
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Upstream Regulation
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Potential in vivo enzymes for site:
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Type
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Enzyme
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Evidence
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Notes
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KINASE
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PLK1 (human)
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siRNA inhibition of enzyme
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Downstream Regulation
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Effect of modification (function):
sumoylation, ubiquitination
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Comments:
inhibits sumoylation but induces ubiquitination of p53
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