Curated Information
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Curated Information Page
PubMed Id: 10753910 
Flynn P, Mellor H, Casamassima A, Parker PJ (2000) Rho GTPase control of protein kinase C-related protein kinase activation by 3-phosphoinositide-dependent protein kinase. J Biol Chem 275, 11064-70 10753910
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Click on the protein name to open the protein page, and on the RSD number to open the site page.
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T774-p - PKN1 (human)
Orthologous residues
PKN1 (human): T774‑p, PKN1 iso2 (human): T780‑p, PKN1 (mouse): T778‑p, PKN1 (rat): T778‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody
 Relevant cell lines - cell types - tissues:  293 (epithelial) [PDK1 (human)], 293 (epithelial) [RHOA (human)], 293 (epithelial) [RHOB (human)]
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE PDK1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PDK1 (human) genetic transfer of constitutively active upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
LY294002 decrease
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced

T816-p - PKN2 (human)
Orthologous residues
PKN2 (human): T816‑p, PKN2 iso2 (human): T800‑p, PKN2 (mouse): T815‑p, PKN2 (rat): T817‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody
 Relevant cell lines - cell types - tissues:  293 (epithelial) [PDK1 (human)], 293 (epithelial) [RHOA (human)], 293 (epithelial) [RHOB (human)]
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PDK1 (human) genetic transfer of constitutively active upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
LY294002 decrease
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced


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