Curated Information
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PubMed Id: 19287004 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
Yu L, et al. (2009) JAK2 and SHP2 Reciprocally Regulate Tyrosine Phosphorylation and Stability of Proapoptotic Protein ASK1. J Biol Chem 284, 13481-8 19287004
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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Y718-p - ASK1 (human)
Orthologous residues
ASK1 (human): Y718‑p, ASK1 iso5 (human): Y798‑p, ASK1 (mouse): Y725‑p, ASK1 (rat): Y689‑p
Characterization
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE JAK2 (human)
KINASE JAK1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE JAK2 (human) mutation in upstream enzyme recognition motif, transfection of wild-type enzyme, co-immunoprecipitation, phospho-antibody, activation of upstream enzyme
PHOSPHATASE SHP-2 (human) transfection of inactive enzyme, transfection of wild-type enzyme, co-immunoprecipitation
 Comments:  "substrate trapping" mutant
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IFN-beta no change compared to control wild-type
TNF no change compared to control
IFN-gamma increase
IFN-beta no change compared to control SOCS1 KO
TNF increase
IFN-gamma increase
IFN-gamma no change compared to control SHP2-KO
Downstream Regulation
 Effect of modification (function):  molecular association, regulation, protein degradation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
SOCS1 (human) Induces molecular association, regulation co-immunoprecipitation
 Comments:  SHP2 dephosphorylation of ASK1 Y798 disrupts binding of ASK1 to SOCS1 and induces apoptosis.

T838-p - ASK1 (human)
Orthologous residues
ASK1 (human): T838‑p, ASK1 iso5 (human): T918‑p, ASK1 (mouse): T845‑p, ASK1 (rat): T809‑p
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TNF increase wild-type
TNF no change compared to control SHP2-KO

Y1007-p - JAK2 (human)
Orthologous residues
JAK2 (human): Y1007‑p, JAK2 (mouse): Y1007‑p, JAK2 (rat): Y1007‑p
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IFN-gamma increase
IFN-gamma increase wild-type
increase SHP2-KO

Y1008-p - JAK2 (human)
Orthologous residues
JAK2 (human): Y1008‑p, JAK2 (mouse): Y1008‑p, JAK2 (rat): Y1008‑p
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IFN-gamma increase
IFN-gamma increase wild-type
increase SHP2-KO

T183-p - JNK1 (human)
Orthologous residues
JNK1 (human): T183‑p, JNK1 iso2 (human): T183‑p, JNK1 iso3 (human): T183‑p, JNK1 (mouse): T183‑p, JNK1 (rat): T183‑p

Y185-p - JNK1 (human)
Orthologous residues
JNK1 (human): Y185‑p, JNK1 iso2 (human): Y185‑p, JNK1 iso3 (human): Y185‑p, JNK1 (mouse): Y185‑p, JNK1 (rat): Y185‑p

T183-p - JNK2 (human)
Orthologous residues
JNK2 (human): T183‑p, JNK2 iso2 (human): T183‑p, JNK2 iso3 (human): T183‑p, JNK2 (mouse): T183‑p, JNK2 (rat): T183‑p
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TNF increase wild-type
TNF no change compared to control SHP2-KO

Y185-p - JNK2 (human)
Orthologous residues
JNK2 (human): Y185‑p, JNK2 iso2 (human): Y185‑p, JNK2 iso3 (human): Y185‑p, JNK2 (mouse): Y185‑p, JNK2 (rat): Y185‑p
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TNF increase wild-type
TNF no change compared to control SHP2-KO

Y546-p - SHP-2 (human)
Orthologous residues
SHP‑2 (human): Y546‑p, SHP‑2 iso2 (human): Y542‑p, SHP‑2 (mouse): Y546‑p, SHP‑2 (rat): Y546‑p
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TNF increase wild-type
TNF no change compared to control SHP2-KO

Y701-p - STAT1 (human)
Orthologous residues
STAT1 (human): Y701‑p, STAT1 (mouse): Y701‑p, STAT1 (rat): Y701‑p
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IFN-gamma increase


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