Curated Information
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Curated Information Page
PubMed Id: 19193766 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Miura K, et al. (2009) EphA2 engages Git1 to suppress Arf6 activity modulating epithelial cell-cell contacts. Mol Biol Cell 20, 1949-59 19193766
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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Y588-p - EphA2 (human) ▲
Orthologous residues
EphA2 (human): Y588‑p, EphA2 (mouse): Y589‑p, EphA2 (rat): Y589‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  293T (epithelial), MDCK (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  dog, human
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Effect of modification (process):  cell adhesion, altered
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Nck1 (human) SH2 Induces molecular association, regulation co-immunoprecipitation

Y594-p - EphA2 (human) ▲
Orthologous residues
EphA2 (human): Y594‑p, EphA2 (mouse): Y595‑p, EphA2 (rat): Y595‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  293T (epithelial), MDCK (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  dog, human

T202-p - ERK1 (human) ▲
Orthologous residues
ERK1 (human): T202‑p, ERK1 (mouse): T203‑p, ERK1 (rat): T203‑p, ERK1 (hamster): T192‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293T (epithelial), MDCK (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  dog, human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGTA increase
Ca(2+) increase
ephrin A1 decrease Ephrin A1 Fc
siRNA ephrin A1 GIT1 (human) inhibit treatment-induced decrease

Y204-p - ERK1 (human) ▲
Orthologous residues
ERK1 (human): Y204‑p, ERK1 (mouse): Y205‑p, ERK1 (rat): Y205‑p, ERK1 (hamster): Y194‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293T (epithelial), MDCK (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  dog, human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGTA increase
Ca(2+) increase
ephrin A1 decrease Ephrin A1 Fc
siRNA ephrin A1 GIT1 (human) inhibit treatment-induced decrease

T185-p - ERK2 (human) ▲
Orthologous residues
ERK2 (human): T185‑p, ERK2 (mouse): T183‑p, ERK2 (rat): T183‑p, ERK2 (chicken): T193‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293T (epithelial), MDCK (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  dog, human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGTA increase
Ca(2+) increase
ephrin A1 decrease Ephrin A1 Fc
siRNA ephrin A1 GIT1 (human) inhibit treatment-induced decrease

Y187-p - ERK2 (human) ▲
Orthologous residues
ERK2 (human): Y187‑p, ERK2 (mouse): Y185‑p, ERK2 (rat): Y185‑p, ERK2 (chicken): Y195‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293T (epithelial), MDCK (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  dog, human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGTA increase
Ca(2+) increase
ephrin A1 decrease Ephrin A1 Fc
siRNA ephrin A1 GIT1 (human) inhibit treatment-induced decrease

Y383-p - GIT1 (human) ▲
Orthologous residues
GIT1 (human): Y383‑p, GIT1 iso3 (human): Y396‑p, GIT1 (mouse): Y392‑p, GIT1 (rat): Y392‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  293T (epithelial), MDCK (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  dog, human


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