Curated Information
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Curated Information Page
PubMed Id: 1708307 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
Kim HK, et al. (1991) PDGF stimulation of inositol phospholipid hydrolysis requires PLC-gamma 1 phosphorylation on tyrosine residues 783 and 1254. Cell 65, 435-41 1708307
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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Y771-p - PLCG1 (rat) ▲
Orthologous residues
PLCG1 (human): Y771‑p, PLCG1 iso2 (human): Y771‑p, PLCG1 (mouse): Y771‑p, PLCG1 (rat): Y771‑p, PLCG1 (cow): Y771‑p
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, mutation of modification site, phosphopeptide mapping, western blotting
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PDGF increase
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced
 Comments:  Y771F mutation completely blocked activation while Y774F enhanced, and Y1253F inhibited full activation.

Y783-p - PLCG1 (rat) ▲
Orthologous residues
PLCG1 (human): Y783‑p, PLCG1 iso2 (human): Y783‑p, PLCG1 (mouse): Y783‑p, PLCG1 (rat): Y783‑p, PLCG1 (cow): Y783‑p
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, mutation of modification site, phosphopeptide mapping, western blotting
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PDGF increase
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced
 Comments:  Y771F mutation completely blocked activation while Y774F enhanced, and Y1253F inhibited full activation.

Y1253-p - PLCG1 (rat) ▲
Orthologous residues
PLCG1 (human): Y1253‑p, PLCG1 iso2 (human): Y1254‑p, PLCG1 (mouse): Y1253‑p, PLCG1 (rat): Y1253‑p, PLCG1 (cow): Y1254‑p
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, mutation of modification site, phosphopeptide mapping, western blotting
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PDGF increase
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced
 Comments:  Y771F mutation completely blocked activation while Y774F enhanced, and Y1253F inhibited full activation.


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