Curated Information
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Curated Information Page
PubMed Id: 19468302 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Burkard ME, et al. (2009) Plk1 Self-Organization and Priming Phosphorylation of HsCYK-4 at the Spindle Midzone Regulate the Onset of Division in Human Cells. PLoS Biol 7, e1000111 19468302
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S157-p - MgcRacGAP (human)
Orthologous residues
MgcRacGAP (human): S157‑p, MgcRacGAP (mouse): S158‑p, MgcRacGAP (rat): S158‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
 Disease tissue studied:  colorectal cancer, colorectal carcinoma
 Relevant cell lines - cell types - tissues:  HCT116 (intestinal), HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE PLK1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PLK1 (human) pharmacological inhibitor of upstream enzyme, co-immunoprecipitation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
3-MB-PP1 decrease
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Effect of modification (process):  cell cycle regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
ECT2 (human) Induces co-immunoprecipitation

S164-p - MgcRacGAP (human)
Orthologous residues
MgcRacGAP (human): S164‑p, MgcRacGAP (mouse): S165‑p, MgcRacGAP (rat): S165‑p
Characterization
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE PLK1 (human)

S170-p - MgcRacGAP (human)
Orthologous residues
MgcRacGAP (human): S170‑p, MgcRacGAP (mouse): S171‑p, MgcRacGAP (rat): S171‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
 Disease tissue studied:  colorectal cancer, colorectal carcinoma
 Relevant cell lines - cell types - tissues:  HCT116 (intestinal), HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE PLK1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PLK1 (human) pharmacological inhibitor of upstream enzyme, activation of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase
3-MB-PP1 decrease
Downstream Regulation
 Effect of modification (process):  cell cycle regulation

S214-p - MgcRacGAP (human)
Orthologous residues
MgcRacGAP (human): S214‑p, MgcRacGAP (mouse): S215‑p, MgcRacGAP (rat): S214‑p
Characterization
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE PLK1 (human)

T260-p - MgcRacGAP (human)
Orthologous residues
MgcRacGAP (human): T260‑p, MgcRacGAP (mouse): A261‑p, MgcRacGAP (rat): A260‑p
Characterization
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE PLK1 (human)


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