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Orthologous residues
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Rb (human): K873‑a, Rb (mouse): K866‑a, Rb (rat): K865‑a
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Characterization
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Methods used to characterize site in vivo:
immunoprecipitation, modification-specific antibody, mutation of modification site, western blotting
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Disease tissue studied:
bone cancer, brain cancer, glioblastoma multiforme, glioma
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Relevant cell lines - cell types - tissues:
293 (epithelial), Saos-2 (bone cell), T98G (glial), U2OS (bone cell)
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Cellular systems studied:
cell lines
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Species studied:
human
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Enzymes shown to modify site in vitro:
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Downstream Regulation
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Effect of modification (function):
phosphorylation
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Effect of modification (process):
cell cycle regulation
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Comments:
reduced level of CDK-dependent phosphorylation of Rb KK873/874QQ mutant
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