Curated Information
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Curated Information Page
PubMed Id: 19336760 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Sulahian R, Cleaver O, Huang LJ (2009) Ligand-induced EpoR internalization is mediated by JAK2 and p85 and is impaired by mutations responsible for primary familial and congenital polycythemia. Blood 113, 5287-97 19336760
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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Y367-p - EpoR (mouse)
Orthologous residues
EpoR (human): Y368‑p, EpoR (mouse): Y367‑p, EpoR (rat): Y367‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  BaF3 ('B lymphocyte, precursor'), gamma 2a (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse

Y425-p - EpoR (mouse)
Orthologous residues
EpoR (human): Y426‑p, EpoR (mouse): Y425‑p, EpoR (rat): Y425‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  BaF3 ('B lymphocyte, precursor'), gamma 2a (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse

Y453-p - EpoR (mouse)
Orthologous residues
EpoR (human): Y454‑p, EpoR (mouse): Y453‑p, EpoR (rat): Y453‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  BaF3 ('B lymphocyte, precursor'), gamma 2a (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE JAK2 (human) transfection of constitutively active enzyme, transfection of dominant-negative enzyme
Downstream Regulation
 Effect of modification (function):  molecular association, regulation, receptor internalization, altered
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
PIK3R1 (mouse) Induces co-immunoprecipitation
PIK3R2 (mouse) Induces co-immunoprecipitation
 Comments:  required for optimal PIK3R1/2 binding
Associated Diseases
Diseases: Alterations: Comments:
PFCP deletion of site

Y455-p - EpoR (mouse)
Orthologous residues
EpoR (human): Y456‑p, EpoR (mouse): Y455‑p, EpoR (rat): Y455‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  BaF3 ('B lymphocyte, precursor'), gamma 2a (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE JAK2 (human) transfection of constitutively active enzyme, transfection of dominant-negative enzyme
Downstream Regulation
 Effect of modification (function):  molecular association, regulation, receptor internalization, altered
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
PIK3R1 (mouse) Induces co-immunoprecipitation
PIK3R2 (mouse) Induces co-immunoprecipitation
 Comments:  required for optimal PIK3R1/2 binding
Associated Diseases
Diseases: Alterations: Comments:
PFCP deletion of site

Y467-p - EpoR (mouse)
Orthologous residues
EpoR (human): Y468‑p, EpoR (mouse): Y467‑p, EpoR (rat): Y467‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  BaF3 ('B lymphocyte, precursor'), gamma 2a (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse

Y484-p - EpoR (mouse)
Orthologous residues
EpoR (human): Y485‑p, EpoR (mouse): Y484‑p, EpoR (rat): Y484‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  BaF3 ('B lymphocyte, precursor'), gamma 2a (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse

Y488-p - EpoR (mouse)
Orthologous residues
EpoR (human): Y489‑p, EpoR (mouse): Y488‑p, EpoR (rat): Y488‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  BaF3 ('B lymphocyte, precursor'), gamma 2a (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse

Y503-p - EpoR (mouse)
Orthologous residues
EpoR (human): Y504‑p, EpoR (mouse): Y503‑p, EpoR (rat): Y503‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  BaF3 ('B lymphocyte, precursor'), gamma 2a (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE JAK2 (human) transfection of constitutively active enzyme, transfection of dominant-negative enzyme
Downstream Regulation
 Effect of modification (function):  molecular association, regulation, receptor internalization, altered
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
PIK3R2 (mouse) Induces co-immunoprecipitation
PIK3R1 (mouse) Induces co-immunoprecipitation
Associated Diseases
Diseases: Alterations: Comments:
PFCP deletion of site


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