Curated Information
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Curated Information Page
PubMed Id: 15522277 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Markou T, Hadzopoulou-Cladaras M, Lazou A (2004) Phenylephrine induces activation of CREB in adult rat cardiac myocytes through MSK1 and PKA signaling pathways. J Mol Cell Cardiol 37, 1001-11 15522277
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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T581-p - MSK1 (human)
Orthologous residues
MSK1 (human): T581‑p, MSK1 (mouse): T645‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  myocyte-heart
 Cellular systems studied:  primary cells
 Species studied:  rat
 Comments:  rat MSK1 curated as human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phenylephrine increase
Ro31-8220 phenylephrine no effect upon treatment-induced increase
H-89 phenylephrine no effect upon treatment-induced increase
8-Rp-cAMP phenylephrine no effect upon treatment-induced increase

S133-p - CREB (rat)
Orthologous residues
CREB (human): S133‑p, CREB iso2 (human): S119‑p, CREB (mouse): S133‑p, CREB (rat): S133‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  myocyte-heart
 Cellular systems studied:  primary cells
 Species studied:  rat
 Comments:  rat MSK1 curated as human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phenylephrine increase
prazosin phenylephrine inhibit treatment-induced increase
propranolol phenylephrine inhibit treatment-induced increase
prazosin, propranolol phenylephrine inhibit treatment-induced increase
Ro31-8220 phenylephrine inhibit treatment-induced increase
H-89 phenylephrine inhibit treatment-induced increase
SB203580 phenylephrine inhibit treatment-induced increase
8-Rp-cAMP phenylephrine inhibit treatment-induced increase
forskolin phenylephrine inhibit treatment-induced increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Effect of modification (process):  transcription, induced
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
CBP (rat) Induces molecular association, regulation transcription, altered co-immunoprecipitation


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