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Orthologous residues
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MEKK3 (human): S526‑p, MEKK3 (mouse): S526‑p, MEKK3 (rat): S526‑p
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Characterization
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Methods used to characterize site in vivo:
mutation of modification site, phospho-antibody
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Relevant cell lines - cell types - tissues:
293 (epithelial)
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Cellular systems studied:
cell lines
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Species studied:
human
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Upstream Regulation
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Potential in vivo enzymes for site:
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Type
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Enzyme
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Evidence
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Notes
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KINASE
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MKK3 (mouse)
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transfection of inactive enzyme
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Downstream Regulation
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Effect of modification (function):
enzymatic activity, induced
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Modification regulates interactions with:
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Interacting molecule
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Interacting domains
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Effect
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Consequences (function)
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Consequences (process)
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Detection assays
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14-3-3 epsilon (mouse)
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Induces
|
|
|
co-immunoprecipitation
|
|
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Comments:
interaction with 14-3-3 epsilon prevents dephosphorylation of S526. critical site for activation of ERK, p38 and JNK kinases.
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