Curated Information
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Curated Information Page
PubMed Id: 16407301 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Fritz A, et al. (2006) Phosphorylation of serine 526 is required for MEKK3 activity, and association with 14-3-3 blocks dephosphorylation. J Biol Chem 281, 6236-45 16407301
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S526-p - MEKK3 (mouse)
Orthologous residues
MEKK3 (human): S526‑p, MEKK3 iso2 (human): S557‑p, MEKK3 (mouse): S526‑p, MEKK3 (rat): S526‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, phospho-antibody
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE MKK3 (mouse) transfection of inactive enzyme
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
14-3-3 epsilon (mouse) Induces co-immunoprecipitation
 Comments:  interaction with 14-3-3 epsilon prevents dephosphorylation of S526. critical site for activation of ERK, p38 and JNK kinases.


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