Curated Information
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Curated Information Page
PubMed Id: 7588273 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
Rocchi S, Tartare-Deckert S, Mothe I, Van Obberghen E (1995) Identification by mutation of the tyrosine residues in the insulin receptor substrate-1 affecting association with the tyrosine phosphatase 2C and phosphatidylinositol 3-kinase. Endocrinology 136, 5291-7 7588273
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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Y608-p - IRS1 (rat) ▲
Orthologous residues
IRS1 (human): Y612‑p, IRS1 (mouse): Y608‑p, IRS1 (rat): Y608‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  293E (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
PHOSPHATASE SHP-2 (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
PIK3R1 (human) SH2 Induces enzymatic activity, induced co-immunoprecipitation

Y895-p - IRS1 (rat) ▲
Orthologous residues
IRS1 (human): Y896‑p, IRS1 (mouse): Y891‑p, IRS1 (rat): Y895‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  293E (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
PHOSPHATASE SHP-2 (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase

Y1172-p - IRS1 (rat) ▲
Orthologous residues
IRS1 (human): Y1179‑p, IRS1 (mouse): Y1173‑p, IRS1 (rat): Y1172‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  293E (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
SHP-2 (human) SH2 Induces molecular association, regulation co-immunoprecipitation, in vitro

Y1222-p - IRS1 (rat) ▲
Orthologous residues
IRS1 (human): Y1229‑p, IRS1 (mouse): Y1220‑p, IRS1 (rat): Y1222‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  293E (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
SHP-2 (human) SH2 Induces molecular association, regulation co-immunoprecipitation, in vitro


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