Curated Information
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Curated Information Page
PubMed Id: 16260496 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Yamaguchi T, et al. (2005) Phosphorylation by Cdk1 induces Plk1-mediated vimentin phosphorylation during mitosis. J Cell Biol 171, 431-6 16260496
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S56-p - vimentin (mouse) ▲
Orthologous residues
vimentin (human): S56‑p, vimentin (mouse): S56‑p, vimentin (rat): S56‑p, vimentin (hamster): S55‑p, vimentin (cow): S56‑p
Characterization
 Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody
 Relevant cell lines - cell types - tissues:  COS (fibroblast) [PLK1 (human)], COS (fibroblast) [vimentin (mouse)], T24 (bladder cell) [PLK1 (human)], T24 (bladder cell) [vimentin (mouse)], U251 (glial) [PLK1 (human)], U251 (glial) [vimentin (mouse)]
 Cellular systems studied:  cell lines
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE CDK1 (mouse)
Downstream Regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
PLK1 (human) Induces activation far-Western

S72-p - vimentin (mouse) ▲
Orthologous residues
vimentin (human): S72‑p, vimentin (mouse): S72‑p, vimentin (rat): S72‑p, vimentin (hamster): S71‑p, vimentin (cow): S72‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody
 Relevant cell lines - cell types - tissues:  COS (fibroblast) [PLK1 (human)], COS (fibroblast) [vimentin (mouse)], T24 (bladder cell) [PLK1 (human)], T24 (bladder cell) [vimentin (mouse)], U251 (glial) [PLK1 (human)], U251 (glial) [vimentin (mouse)]
 Cellular systems studied:  cell lines

S73-p - vimentin (mouse) ▲
Orthologous residues
vimentin (human): S73‑p, vimentin (mouse): S73‑p, vimentin (rat): S73‑p, vimentin (hamster): S72‑p, vimentin (cow): G73‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody
 Relevant cell lines - cell types - tissues:  COS (fibroblast) [PLK1 (human)], COS (fibroblast) [vimentin (mouse)], T24 (bladder cell) [PLK1 (human)], T24 (bladder cell) [vimentin (mouse)], U251 (glial) [PLK1 (human)], U251 (glial) [vimentin (mouse)]
 Cellular systems studied:  cell lines

S83-p - vimentin (mouse) ▲
Orthologous residues
vimentin (human): S83‑p, vimentin (mouse): S83‑p, vimentin (rat): S83‑p, vimentin (hamster): S82‑p, vimentin (cow): S83‑p
Characterization
 Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody
 Relevant cell lines - cell types - tissues:  COS (fibroblast) [PLK1 (human)], COS (fibroblast) [vimentin (mouse)], T24 (bladder cell) [PLK1 (human)], T24 (bladder cell) [vimentin (mouse)], U251 (glial) [PLK1 (human)], U251 (glial) [vimentin (mouse)]
 Cellular systems studied:  cell lines
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE PLK1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PLK1 (human) transfection of constitutively active enzyme, transfection of inactive enzyme


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