Curated Information
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Curated Information Page
PubMed Id: 9349507 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Knippschild U, et al. (1997) p53 is phosphorylated in vitro and in vivo by the delta and epsilon isoforms of casein kinase 1 and enhances the level of casein kinase 1 delta in response to topoisomerase-directed drugs. Oncogene 15, 1727-36 9349507
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S4-p - p53 (mouse)
Orthologous residues
p53 (human): P4‑p, p53 (mouse): S4‑p, p53 iso2 (mouse): S7‑p, p53 (rat): S4‑p, p53 (rabbit): S4‑p, p53 (monkey): P4‑p
Characterization
 Methods used to characterize site in vivo 2D analysis, [32P] bio-synthetic labeling, immunoprecipitation, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  COS (fibroblast), SV3T3 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse, rat
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE CK1D (human)
KINASE CK1E (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CK1D (human) pharmacological inhibitor of upstream enzyme, co-immunoprecipitation, transfection of wild-type enzyme
KINASE CK1E (human) pharmacological inhibitor of upstream enzyme, co-immunoprecipitation, transfection of wild-type enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IC261 decrease

S6-p - p53 (mouse)
Orthologous residues
p53 (human): S6‑p, p53 (mouse): S6‑p, p53 iso2 (mouse): S9‑p, p53 (rat): S6‑p, p53 (rabbit): S6‑p, p53 (monkey): S6‑p
Characterization
 Methods used to characterize site in vivo 2D analysis, [32P] bio-synthetic labeling, immunoprecipitation, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  COS (fibroblast), SV3T3 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse, rat
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE CK1D (human)
KINASE CK1E (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CK1E (human) pharmacological inhibitor of upstream enzyme, co-immunoprecipitation, transfection of wild-type enzyme
KINASE CK1D (human) pharmacological inhibitor of upstream enzyme, co-immunoprecipitation, transfection of wild-type enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IC261 decrease

S9-p - p53 (mouse)
Orthologous residues
p53 (human): S9‑p, p53 (mouse): S9‑p, p53 iso2 (mouse): S12‑p, p53 (rat): S9‑p, p53 (rabbit): S9‑p, p53 (monkey): S9‑p
Characterization
 Methods used to characterize site in vivo 2D analysis, [32P] bio-synthetic labeling, immunoprecipitation, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  COS (fibroblast), SV3T3 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse, rat
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE CK1E (human)
KINASE CK1D (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CK1E (human) pharmacological inhibitor of upstream enzyme, co-immunoprecipitation, transfection of wild-type enzyme
KINASE CK1D (human) pharmacological inhibitor of upstream enzyme, co-immunoprecipitation, transfection of wild-type enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IC261 decrease


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