Curated Information
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PubMed Id: 15574771 
Topisirovic I, Ruiz-Gutierrez M, Borden KL (2004) Phosphorylation of the eukaryotic translation initiation factor eIF4E contributes to its transformation and mRNA transport activities. Cancer Res 64, 8639-42 15574771
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
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S209-p - eIF4E (human)
Orthologous residues
eIF4E (human): S209‑p, eIF4E iso4 (human): S237‑p, eIF4E (mouse): S209‑p, eIF4E (rat): S209‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
CGP57380 decrease
Downstream Regulation
 Effect of modification (process):  cell growth, altered
 Comments:  S209A/T210A mutant showed reduced transport of cyclin D1 mRNA from nucleus to cytoplasm. eiF4E cellular distribution not affected by phosphorylation.

T210-p - eIF4E (human)
Orthologous residues
eIF4E (human): T210‑p, eIF4E iso4 (human): T238‑p, eIF4E (mouse): T210‑p, eIF4E (rat): T210‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
CGP57380 decrease
Downstream Regulation
 Effect of modification (process):  cell growth, altered
 Comments:  S209A/T210A mutant showed reduced transport of cyclin D1 mRNA from nucleus to cytoplasm. eiF4E cellular distribution not affected by phosphorylation.


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