Curated Information
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Curated Information Page
PubMed Id: 1314164 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
Kashishian A, Kazlauskas A, Cooper JA (1992) Phosphorylation sites in the PDGF receptor with different specificities for binding GAP and PI3 kinase in vivo. EMBO J 11, 1373-82 1314164
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
Download Sites

Y716-p - PDGFRb (human) ▲
Orthologous residues
PDGFRb (human): Y716‑p, PDGFRb (mouse): Y715‑p, PDGFRb (rat): Y715‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  epithelial
 Cellular systems studied:  cell lines
 Species studied:  dog
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PDGF increase

Y740-p - PDGFRb (human) ▲
Orthologous residues
PDGFRb (human): Y740‑p, PDGFRb (mouse): Y739‑p, PDGFRb (rat): Y739‑p
Characterization
 Methods used to characterize site in vivo:  2D analysis, immunoprecipitation, mutation of modification site, phosphopeptide mapping, western blotting
 Relevant cell lines - cell types - tissues:  epithelial
 Cellular systems studied:  cell lines
 Species studied:  dog
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PDGF increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
PIK3R1 (human) Induces in vitro, functional assay
 Comments:  PIK3R1 kinase activity assay

Y751-p - PDGFRb (human) ▲
Orthologous residues
PDGFRb (human): Y751‑p, PDGFRb (mouse): Y750‑p, PDGFRb (rat): Y750‑p
Characterization
 Methods used to characterize site in vivo:  2D analysis, immunoprecipitation, mutation of modification site, phosphopeptide mapping, western blotting
 Relevant cell lines - cell types - tissues:  epithelial
 Cellular systems studied:  cell lines
 Species studied:  dog
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PDGF increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
PIK3R1 (human) Induces in vitro, functional assay
 Comments:  PIK3R1 kinase activity assay

Y763-p - PDGFRb (human) ▲
Orthologous residues
PDGFRb (human): Y763‑p, PDGFRb (mouse): Y762‑p, PDGFRb (rat): Y762‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  epithelial
 Cellular systems studied:  cell lines
 Species studied:  dog
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PDGF increase

Y771-p - PDGFRb (human) ▲
Orthologous residues
PDGFRb (human): Y771‑p, PDGFRb (mouse): Y770‑p, PDGFRb (rat): Y770‑p
Characterization
 Methods used to characterize site in vivo:  2D analysis, immunoprecipitation, mutation of modification site, phosphopeptide mapping, western blotting
 Relevant cell lines - cell types - tissues:  epithelial
 Cellular systems studied:  cell lines
 Species studied:  dog
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PDGF increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
RASA1 (human) Induces co-immunoprecipitation

Y775-p - PDGFRb (human) ▲
Orthologous residues
PDGFRb (human): Y775‑p, PDGFRb (mouse): Y774‑p, PDGFRb (rat): Y774‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  epithelial
 Cellular systems studied:  cell lines
 Species studied:  dog
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PDGF increase

Y778-p - PDGFRb (human) ▲
Orthologous residues
PDGFRb (human): Y778‑p, PDGFRb (mouse): Y777‑p, PDGFRb (rat): Y777‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  epithelial
 Cellular systems studied:  cell lines
 Species studied:  dog
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PDGF increase

Y857-p - PDGFRb (human) ▲
Orthologous residues
PDGFRb (human): Y857‑p, PDGFRb (mouse): Y856‑p, PDGFRb (rat): Y856‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  epithelial
 Cellular systems studied:  cell lines
 Species studied:  dog
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PDGF increase


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