Curated Information
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Curated Information Page
PubMed Id: 8910602 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
Hao M, et al. (1996) Mutation of phosphoserine 389 affects p53 function in vivo. J Biol Chem 271, 29380-5 8910602
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
Download Sites

S4-p - p53 (mouse)
Orthologous residues
p53 (human): P4‑p, p53 (mouse): S4‑p, p53 iso2 (mouse): S7‑p, p53 (rat): S4‑p, p53 (rabbit): S4‑p, p53 (monkey): P4‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse

S6-p - p53 (mouse)
Orthologous residues
p53 (human): S6‑p, p53 (mouse): S6‑p, p53 iso2 (mouse): S9‑p, p53 (rat): S6‑p, p53 (rabbit): S6‑p, p53 (monkey): S6‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse

S9-p - p53 (mouse)
Orthologous residues
p53 (human): S9‑p, p53 (mouse): S9‑p, p53 iso2 (mouse): S12‑p, p53 (rat): S9‑p, p53 (rabbit): S9‑p, p53 (monkey): S9‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse

S15-p - p53 (mouse)
Orthologous residues
p53 (human): S15‑p, p53 (mouse): S15‑p, p53 iso2 (mouse): S18‑p, p53 (rat): S15‑p, p53 (rabbit): S15‑p, p53 (monkey): S15‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse

S34-p - p53 (mouse)
Orthologous residues
p53 (human): S37‑p, p53 (mouse): S34‑p, p53 iso2 (mouse): S37‑p, p53 (rat): S39‑p, p53 (rabbit): N37‑p, p53 (monkey): S37‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse

S309-p - p53 (mouse)
Orthologous residues
p53 (human): S315‑p, p53 (mouse): S309‑p, p53 iso2 (mouse): S312‑p, p53 (rat): S313‑p, p53 (rabbit): S313‑p, p53 (monkey): S315‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse

S386-p - p53 (mouse)
Orthologous residues
p53 (human): S392‑p, p53 (mouse): S386‑p, p53 iso2 (mouse): , p53 (rat): S390‑p, p53 (rabbit): S390‑p, p53 (monkey): S392‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
CCNE1 (human) increase Overexpression of cyclin E induced specific phosphorylation of S389 in high density cells. Phosphoylation at S389 may induce other serine residue phosphorylation.
Downstream Regulation
 Effect of modification (function):  activity, induced
 Effect of modification (process):  transcription, induced
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
DNA Induces activity, induced transcription, altered in vitro, pull-down assay
 Comments:  induced by cyclin E oveerexpression in high density cells


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