Curated Information
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PubMed Id: 8910602 
Hao M, et al. (1996) Mutation of phosphoserine 389 affects p53 function in vivo. J Biol Chem 271, 29380-5 8910602
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
Click on the protein name to open the protein page, and on the RSD number to open the site page.
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S4-p - p53 (mouse)
Orthologous residues
p53 (human): P4‑p, p53 (mouse): S4‑p, p53 iso2 (mouse): S7‑p, p53 (rat): S4‑p, p53 (rabbit): S4‑p, p53 (monkey): P4‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse

S6-p - p53 (mouse)
Orthologous residues
p53 (human): S6‑p, p53 (mouse): S6‑p, p53 iso2 (mouse): S9‑p, p53 (rat): S6‑p, p53 (rabbit): S6‑p, p53 (monkey): S6‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse

S9-p - p53 (mouse)
Orthologous residues
p53 (human): S9‑p, p53 (mouse): S9‑p, p53 iso2 (mouse): S12‑p, p53 (rat): S9‑p, p53 (rabbit): S9‑p, p53 (monkey): S9‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse

S15-p - p53 (mouse)
Orthologous residues
p53 (human): S15‑p, p53 (mouse): S15‑p, p53 iso2 (mouse): S18‑p, p53 (rat): S15‑p, p53 (rabbit): S15‑p, p53 (monkey): S15‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse

S34-p - p53 (mouse)
Orthologous residues
p53 (human): S37‑p, p53 (mouse): S34‑p, p53 iso2 (mouse): S37‑p, p53 (rat): S39‑p, p53 (rabbit): N37‑p, p53 (monkey): S37‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse

S309-p - p53 (mouse)
Orthologous residues
p53 (human): S315‑p, p53 (mouse): S309‑p, p53 iso2 (mouse): S312‑p, p53 (rat): S313‑p, p53 (rabbit): S313‑p, p53 (monkey): S315‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse

S386-p - p53 (mouse)
Orthologous residues
p53 (human): S392‑p, p53 (mouse): S386‑p, p53 iso2 (mouse): , p53 (rat): S390‑p, p53 (rabbit): S390‑p, p53 (monkey): S392‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
CCNE1 (human) increase Overexpression of cyclin E induced specific phosphorylation of S389 in high density cells. Phosphoylation at S389 may induce other serine residue phosphorylation.
Downstream Regulation
 Effect of modification (function):  activity, induced
 Effect of modification (process):  transcription, induced
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
DNA Induces activity, induced transcription, altered pull-down assay, in vitro
 Comments:  induced by cyclin E oveerexpression in high density cells


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