Curated Information
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Curated Information Page
PubMed Id: 10558990 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Bonni A, et al. (1999) Cell survival promoted by the Ras-MAPK signaling pathway by transcription-dependent and -independent mechanisms. Science 286, 1358-62 10558990
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S75-p - BAD (human) ▲
Orthologous residues
BAD (human): S75‑p, BAD (mouse): S112‑p, BAD (rat): S113‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'neuron, cerebellar granule'-brain, 293T (epithelial)
 Cellular systems studied:  cell lines, primary cultured cells
 Species studied:  human, rat
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE RSK2 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE RSK2 (human) transfection of wild-type enzyme, transfection of inactive enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
BDNF increase
PD98059 BDNF inhibit treatment-induced increase
Downstream Regulation
 Effect of modification (function):  activation
 Effect of modification (process):  apoptosis, inhibited

S99-p - BAD (human) ▲
Orthologous residues
BAD (human): S99‑p, BAD (mouse): S136‑p, BAD (rat): S137‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'neuron, cerebellar granule'-brain, 293T (epithelial)
 Cellular systems studied:  cell lines, primary cultured cells
 Species studied:  human, rat
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE Akt1 (human)
KINASE RSK2 (human)

S133-p - CREB (human) ▲
Orthologous residues
CREB (human): S133‑p, CREB iso2 (human): S119‑p, CREB (mouse): S133‑p, CREB (rat): S133‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'neuron, cerebellar granule'-brain
 Cellular systems studied:  primary cultured cells
 Species studied:  rat
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
BDNF increase
PD98059 BDNF inhibit treatment-induced increase
LY294002 BDNF no effect upon treatment-induced increase
Downstream Regulation
 Effect of modification (function):  activation
 Effect of modification (process):  apoptosis, inhibited

T202-p - ERK1 (human) ▲
Orthologous residues
ERK1 (human): T202‑p, ERK1 (mouse): T203‑p, ERK1 (rat): T203‑p, ERK1 (hamster): T192‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'neuron, cerebellar granule'-brain
 Cellular systems studied:  primary cultured cells
 Species studied:  rat
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
BDNF increase
PD98059 BDNF inhibit treatment-induced increase
LY294002 BDNF no effect upon treatment-induced increase

Y204-p - ERK1 (human) ▲
Orthologous residues
ERK1 (human): Y204‑p, ERK1 (mouse): Y205‑p, ERK1 (rat): Y205‑p, ERK1 (hamster): Y194‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'neuron, cerebellar granule'-brain
 Cellular systems studied:  primary cultured cells
 Species studied:  rat
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
BDNF increase
PD98059 BDNF inhibit treatment-induced increase
LY294002 BDNF no effect upon treatment-induced increase

T185-p - ERK2 (human) ▲
Orthologous residues
ERK2 (human): T185‑p, ERK2 (mouse): T183‑p, ERK2 (rat): T183‑p, ERK2 (chicken): T193‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'neuron, cerebellar granule'-brain
 Cellular systems studied:  primary cultured cells
 Species studied:  rat
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
BDNF increase
PD98059 BDNF inhibit treatment-induced increase
LY294002 BDNF no effect upon treatment-induced increase

Y187-p - ERK2 (human) ▲
Orthologous residues
ERK2 (human): Y187‑p, ERK2 (mouse): Y185‑p, ERK2 (rat): Y185‑p, ERK2 (chicken): Y195‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'neuron, cerebellar granule'-brain
 Cellular systems studied:  primary cultured cells
 Species studied:  rat
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
BDNF increase
PD98059 BDNF inhibit treatment-induced increase
LY294002 BDNF no effect upon treatment-induced increase


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