Curated Information
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Curated Information Page
PubMed Id: 10202145 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Shieh SY, Taya Y, Prives C (1999) DNA damage-inducible phosphorylation of p53 at N-terminal sites including a novel site, Ser20, requires tetramerization. EMBO J 18, 1815-23 10202145
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S15-p - p53 (human) ▲
Orthologous residues
p53 (human): S15‑p, p53 (mouse): S15‑p, p53 iso2 (mouse): S18‑p, p53 (rat): S15‑p, p53 (rabbit): S15‑p, p53 (monkey): S15‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
 Disease tissue studied:  lymphoma, anaplastic large cell lymphoma
 Relevant cell lines - cell types - tissues:  CEM (T lymphocyte), HT-29 (intestinal), LNCaP (prostate cell)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
UV increase
ionizing radiation increase

S20-p - p53 (human) ▲
Orthologous residues
p53 (human): S20‑p, p53 (mouse): S20‑p, p53 iso2 (mouse): S23‑p, p53 (rat): S20‑p, p53 (rabbit): S20‑p, p53 (monkey): S20‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
 Disease tissue studied:  lymphoma, anaplastic large cell lymphoma
 Relevant cell lines - cell types - tissues:  CEM (T lymphocyte), HT-29 (intestinal), LNCaP (prostate cell)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing radiation increase The tetramerization domain is required for phosphorylation of p53 at S20 and S33.
UV increase The tetramerization domain is required for phosphorylation of p53 at S20 and S33.

S33-p - p53 (human) ▲
Orthologous residues
p53 (human): S33‑p, p53 (mouse): P33‑p, p53 iso2 (mouse): P36‑p, p53 (rat): P33‑p, p53 (rabbit): T33‑p, p53 (monkey): S33‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
 Disease tissue studied:  lymphoma, anaplastic large cell lymphoma
 Relevant cell lines - cell types - tissues:  CEM (T lymphocyte), HT-29 (intestinal), LNCaP (prostate cell)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
UV increase The tetramerization domain is required for phosphorylation of p53 at S20 and S33.
ionizing radiation increase The tetramerization domain is required for phosphorylation of p53 at S20 and S33.


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