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PubMed Id: 9030583

This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus^®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus^®, click here.

Tingley WG, et al. (1997) Characterization of protein kinase A and protein kinase C phosphorylation of the N-methyl-D-aspartate receptor NR1 subunit using phosphorylation site-specific antibodies. J Biol Chem 272, 5157-66 9030583

Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.

S890-p - NMDAR1 (rat)

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Orthologous residues

NMDAR1 (human): S890‑p, NMDAR1 iso2 (human): ‑, NMDAR1 iso5 (human): S911‑p, NMDAR1 (mouse): S890‑p, NMDAR1 iso2 (mouse): ‑, NMDAR1 iso3 (mouse): S911‑p, NMDAR1 (rat): S890‑p

Characterization

Methods used to characterize site in vivo: phospho-antibody

Relevant cell lines - cell types - tissues: 'brain, hippocampus', 293 (epithelial), QT-6 (fibroblast)

Cellular systems studied: cell lines, tissue

Species studied: human, quail, rat

Comments: transfected 293 and QT-6 cells, and hippocampal slices

Enzymes shown to modify site in vitro:

Type	Enzyme
KINASE	PKCA (rat)

Upstream Regulation

Treatments, proteins and their effect on site modification:

Treatments	Referenced Treatments	Manipulated Protein	Referenced Protein	Effect	Notes
phorbol ester				increase
forskolin				no change compared to control

Downstream Regulation

Effect of modification (function): intracellular localization

S896-p - NMDAR1 (rat)

▲

Orthologous residues

NMDAR1 (human): S896‑p, NMDAR1 iso2 (human): ‑, NMDAR1 iso5 (human): S917‑p, NMDAR1 (mouse): S896‑p, NMDAR1 iso2 (mouse): ‑, NMDAR1 iso3 (mouse): S917‑p, NMDAR1 (rat): S896‑p

Characterization

Methods used to characterize site in vivo: phospho-antibody

Relevant cell lines - cell types - tissues: 'brain, hippocampus', 293 (epithelial), QT-6 (fibroblast)

Cellular systems studied: cell lines, tissue

Species studied: human, quail, rat

Comments: transfected 293 and QT-6 cells, and hippocampal slices

Enzymes shown to modify site in vitro:

Type	Enzyme
KINASE	PKCA (rat)

S897-p - NMDAR1 (rat)

▲

Orthologous residues

NMDAR1 (human): S897‑p, NMDAR1 iso2 (human): ‑, NMDAR1 iso5 (human): S918‑p, NMDAR1 (mouse): S897‑p, NMDAR1 iso2 (mouse): ‑, NMDAR1 iso3 (mouse): S918‑p, NMDAR1 (rat): S897‑p

Characterization

Methods used to characterize site in vivo: phospho-antibody

Relevant cell lines - cell types - tissues: 'brain, hippocampus', 293 (epithelial), QT-6 (fibroblast)

Cellular systems studied: cell lines, tissue

Species studied: human, quail, rat

Comments: transfected 293 and QT-6 cells, and hippocampal slices

Enzymes shown to modify site in vitro:

Type	Enzyme
KINASE	PKACa (cow)

Upstream Regulation

Treatments, proteins and their effect on site modification:

Treatments	Referenced Treatments	Manipulated Protein	Referenced Protein	Effect	Notes
phorbol ester				no change compared to control
forskolin				increase

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