Curated Information
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Curated Information Page
PubMed Id: 11053428 
Leung IW, Lassam N (2001) The kinase activation loop is the key to mixed lineage kinase-3 activation via both autophosphorylation and hematopoietic progenitor kinase 1 phosphorylation. J Biol Chem 276, 1961-7 11053428
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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T261-p - MKK4 (human)
Orthologous residues
MKK4 (human): T261‑p, MKK4 (mouse): T259‑p, MKK4 (rat): T259‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody
 Relevant cell lines - cell types - tissues:  293 (epithelial) [MKK4 (human)], 293 (epithelial) [MLK3 (human)]
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE MLK3 (human) transfection of inactive enzyme
KINASE MLK3 (human) transfection of wild-type enzyme

T277-p - MLK3 (human)
Orthologous residues
MLK3 (human): T277‑p, MLK3 (mouse): T278‑p, MLK3 (rat): T278‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site
 Relevant cell lines - cell types - tissues:  293 (epithelial), HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE MLK3 (human)
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced

S281-p - MLK3 (human)
Orthologous residues
MLK3 (human): S281‑p, MLK3 (mouse): S282‑p, MLK3 (rat): S282‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site
 Relevant cell lines - cell types - tissues:  293 (epithelial), HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE MLK3 (human)
KINASE HPK1 (human)
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced


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