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Orthologous residues
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CTCF (human): S612‑p, CTCF (mouse): S612‑p, CTCF (rat): S612‑p, CTCF (chicken): S612‑p
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Characterization
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Methods used to characterize site in vivo:
[32P] bio-synthetic labeling, immunoprecipitation, mutation of modification site, western blotting
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Relevant cell lines - cell types - tissues:
COS (fibroblast)
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Cellular systems studied:
cell lines
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Species studied:
monkey
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Upstream Regulation
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Potential in vivo enzymes for site:
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Type
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Enzyme
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Evidence
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Notes
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KINASE
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CK2-A1 (human)
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transfection of wild-type enzyme
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Downstream Regulation
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Effect of modification (function):
inhibition
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Effect of modification (process):
transcription, altered
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Comments:
Phosphorylation mutant S612A inhibited transcriptional repression of transcription (CAT activity than wild-type CTCF.Phosphorylation mutant S612A showed transcriptional repression (CAT activity) not significantly different from wild-type after co-transfection with CK2.S612A also did not significanty alter growth suppression of COS7 cells in comparison with wild-type.
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