|
Orthologous residues
|
|
PDE3B (human): S318‑p, PDE3B (mouse): S296‑p, PDE3B (rat): S302‑p
|
|
Characterization
|
|
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling, electrophoretic mobility shift, phosphopeptide mapping
|
|
Relevant cell lines - cell types - tissues:
preadipocyte
|
|
Cellular systems studied:
primary cells
|
|
Species studied:
rat
|
|
Upstream Regulation
|
|
Potential in vivo enzymes for site:
|
|
Type
|
Enzyme
|
Evidence
|
Notes
|
|
PHOSPHATASE
|
PPP2CA (rat)
|
co-immunoprecipitation, pharmacological inhibitor of upstream enzyme
|
|
|
|
Treatments, proteins and their effect on site modification:
|
|
Treatments
|
Referenced Treatments
|
Manipulated Protein
|
Referenced Protein
|
Effect
|
Notes
|
|
insulin
|
|
|
|
increase
|
|
|
okadaic acid
|
|
|
|
increase
|
|
|
calyculin A
|
|
|
|
increase
|
|
|
