Curated Information
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Curated Information Page
PubMed Id: 19186949 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Platt MD, Salicioni AM, Hunt DF, Visconti PE (2009) Use of differential isotopic labeling and mass spectrometry to analyze capacitation-associated changes in the phosphorylation status of mouse sperm proteins. J Proteome Res 8, 1431-40 19186949
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
Download Sites

S65-p - AKAP4 (mouse)
Orthologous residues
AKAP4 (human): S65‑p, AKAP4 (mouse): S65‑p, AKAP4 (rat): S64‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  spermatozoa
 Cellular systems studied:  primary cells
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sperm capacitation increase

S226-p - AKAP4 (mouse)
Orthologous residues
AKAP4 (human): S226‑p, AKAP4 (mouse): S226‑p, AKAP4 (rat): S225‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  spermatozoa
 Cellular systems studied:  primary cells
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sperm capacitation increase

S812-p - AKAP4 (mouse)
Orthologous residues
AKAP4 (human): S817‑p, AKAP4 (mouse): S812‑p, AKAP4 (rat): S810‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  spermatozoa
 Cellular systems studied:  primary cells
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sperm capacitation increase

S168-p - C2orf70 (mouse)
Orthologous residues
C2orf70 (human): A169‑p, C2orf70 (mouse): S168‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  spermatozoa
 Cellular systems studied:  primary cells
 Species studied:  mouse

T238-p - CABYR (mouse)
Orthologous residues
CABYR (human): T239‑p, CABYR iso2 (human): T221‑p, CABYR iso3 (human): , CABYR (mouse): T238‑p, CABYR iso2 (mouse):
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  spermatozoa
 Cellular systems studied:  primary cells
 Species studied:  mouse
 Comments:  ambiguous site (within peptides)

S239-p - CABYR (mouse)
Orthologous residues
CABYR (human): S240‑p, CABYR iso2 (human): S222‑p, CABYR iso3 (human): , CABYR (mouse): S239‑p, CABYR iso2 (mouse):
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  spermatozoa
 Cellular systems studied:  primary cells
 Species studied:  mouse
 Comments:  ambiguous site (within peptides)

S324-p - CABYR (mouse)
Orthologous residues
CABYR (human): S339‑p, CABYR iso2 (human): S321‑p, CABYR iso3 (human): , CABYR (mouse): S324‑p, CABYR iso2 (mouse):
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  spermatozoa
 Cellular systems studied:  primary cells
 Species studied:  mouse

S328-p - CABYR (mouse)
Orthologous residues
CABYR (human): S343‑p, CABYR iso2 (human): S325‑p, CABYR iso3 (human): , CABYR (mouse): S328‑p, CABYR iso2 (mouse):
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  spermatozoa
 Cellular systems studied:  primary cells
 Species studied:  mouse

S339-p - CABYR (mouse)
Orthologous residues
CABYR (human): S359‑p, CABYR iso2 (human): S341‑p, CABYR iso3 (human): , CABYR (mouse): S339‑p, CABYR iso2 (mouse):
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  spermatozoa
 Cellular systems studied:  primary cells
 Species studied:  mouse

S342-p - CABYR (mouse)
Orthologous residues
CABYR (human): S362‑p, CABYR iso2 (human): S344‑p, CABYR iso3 (human): , CABYR (mouse): S342‑p, CABYR iso2 (mouse):
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  spermatozoa
 Cellular systems studied:  primary cells
 Species studied:  mouse

S757-p - CCDC136 (mouse)
Orthologous residues
CCDC136 (human): T761‑p, CCDC136 (mouse): S757‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  spermatozoa
 Cellular systems studied:  primary cells
 Species studied:  mouse
 Comments:  ambiguous site (within peptides)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sperm capacitation increase

Y758-p - CCDC136 (mouse)
Orthologous residues
CCDC136 (human): Y762‑p, CCDC136 (mouse): Y758‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  spermatozoa
 Cellular systems studied:  primary cells
 Species studied:  mouse
 Comments:  ambiguous site (within peptides)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sperm capacitation increase

S760-p - CCDC136 (mouse)
Orthologous residues
CCDC136 (human): T764‑p, CCDC136 (mouse): S760‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  spermatozoa
 Cellular systems studied:  primary cells
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sperm capacitation increase

S761-p - CCDC136 (mouse)
Orthologous residues
CCDC136 (human): T765‑p, CCDC136 (mouse): S761‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  spermatozoa
 Cellular systems studied:  primary cells
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sperm capacitation increase

S101-p - CCDC27 (mouse)
Orthologous residues
CCDC27 (human): S143‑p, CCDC27 (mouse): S101‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  spermatozoa
 Cellular systems studied:  primary cells
 Species studied:  mouse

S107-p - CCDC27 (mouse)
Orthologous residues
CCDC27 (human): S149‑p, CCDC27 (mouse): S107‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  spermatozoa
 Cellular systems studied:  primary cells
 Species studied:  mouse

S124-p - DNAI1 (mouse)
Orthologous residues
DNAI1 (human): S131‑p, DNAI1 (mouse): S124‑p, DNAI1 (rat): S124‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  spermatozoa
 Cellular systems studied:  primary cells
 Species studied:  mouse

S127-p - DNAI1 (mouse)
Orthologous residues
DNAI1 (human): S134‑p, DNAI1 (mouse): S127‑p, DNAI1 (rat): S127‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  spermatozoa
 Cellular systems studied:  primary cells
 Species studied:  mouse

S13-p - Fabp9 (mouse)
Orthologous residues
Fabp9 (human): S13‑p, Fabp9 (mouse): S13‑p, Fabp9 (rat): S13‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  spermatozoa
 Cellular systems studied:  primary cells
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sperm capacitation increase

S449-p - FAM154A (mouse)
Orthologous residues
FAM154A (human): S447‑p, FAM154A (mouse): S449‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  spermatozoa
 Cellular systems studied:  primary cells
 Species studied:  mouse

S453-p - FAM154A (mouse)
Orthologous residues
FAM154A (human): S451‑p, FAM154A (mouse): S453‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  spermatozoa
 Cellular systems studied:  primary cells
 Species studied:  mouse

S57-p - Gm7866 (mouse)
Orthologous residues
Gm7866 (mouse): S57‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  spermatozoa
 Cellular systems studied:  primary cells
 Species studied:  mouse
 Comments:  ambiguous site (between proteins)

S5-p - GSTM3 (mouse)
Orthologous residues
GSTM3 (human): S6‑p, GSTM3 (mouse): S5‑p, GSTM3 (rat): S6‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  spermatozoa
 Cellular systems studied:  primary cells
 Species studied:  mouse

Y83-p - HK1 (mouse)
Orthologous residues
HK1 (human): Y27‑p, HK1 (mouse): Y83‑p, HK1 iso3 (mouse): Y26‑p, HK1 (rat): Y27‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  spermatozoa
 Cellular systems studied:  primary cells
 Species studied:  mouse

S464-p - MKK7 (mouse)
Orthologous residues
MKK7 (human): , MKK7 (mouse): S464‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  spermatozoa
 Cellular systems studied:  primary cells
 Species studied:  mouse

S43-p - MLF1 (mouse)
Orthologous residues
MLF1 (human): S43‑p, MLF1 (mouse): S43‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  spermatozoa
 Cellular systems studied:  primary cells
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sperm capacitation increase

S45-p - MLF1 (mouse)
Orthologous residues
MLF1 (human): S45‑p, MLF1 (mouse): S45‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  spermatozoa
 Cellular systems studied:  primary cells
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sperm capacitation increase

Y18-p - NT5C1B (mouse)
Orthologous residues
NT5C1B (human): S17‑p, NT5C1B iso2 (human): S17‑p, NT5C1B iso5 (human): S17‑p, NT5C1B (mouse): Y18‑p, NT5C1B (rat): Y18‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  spermatozoa
 Cellular systems studied:  primary cells
 Species studied:  mouse
 Comments:  ambiguous site (within peptides)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sperm capacitation increase

S19-p - NT5C1B (mouse)
Orthologous residues
NT5C1B (human): S18‑p, NT5C1B iso2 (human): S18‑p, NT5C1B iso5 (human): S18‑p, NT5C1B (mouse): S19‑p, NT5C1B (rat): S19‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  spermatozoa
 Cellular systems studied:  primary cells
 Species studied:  mouse
 Comments:  ambiguous site (within peptides)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sperm capacitation increase

S22-p - NT5C1B (mouse)
Orthologous residues
NT5C1B (human): S21‑p, NT5C1B iso2 (human): S21‑p, NT5C1B iso5 (human): S21‑p, NT5C1B (mouse): S22‑p, NT5C1B (rat): S22‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  spermatozoa
 Cellular systems studied:  primary cells
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sperm capacitation increase

S628-p - ODF2 iso4 (mouse)
Orthologous residues
ODF2 (human): S632‑p, ODF2 iso3 (human): S608‑p, ODF2 iso10 (human): S628‑p, ODF2 (mouse): S632‑p, ODF2 iso4 (mouse): S628‑p, ODF2 (rat): S627‑p, ODF2 iso3 (rat): S613‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  spermatozoa
 Cellular systems studied:  primary cells
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sperm capacitation decrease

S222-p - ODF3 (mouse)
Orthologous residues
ODF3 (human): S222‑p, ODF3 iso2 (human): , ODF3 (mouse): S222‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  spermatozoa
 Cellular systems studied:  primary cells
 Species studied:  mouse

S361-p - RGS22 (mouse)
Orthologous residues
RGS22 (human): S367‑p, RGS22 (mouse): S361‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  spermatozoa
 Cellular systems studied:  primary cells
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sperm capacitation decrease

S371-p - RGS22 (mouse)
Orthologous residues
RGS22 (human): S377‑p, RGS22 (mouse): S371‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  spermatozoa
 Cellular systems studied:  primary cells
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sperm capacitation decrease

S56-p - Slco6c1 (mouse)
Orthologous residues
Slco6c1 (mouse): S56‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  spermatozoa
 Cellular systems studied:  primary cells
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sperm capacitation increase

S59-p - Slco6c1 (mouse)
Orthologous residues
Slco6c1 (mouse): S59‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  spermatozoa
 Cellular systems studied:  primary cells
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sperm capacitation increase

T265-p - SPACA1 (mouse)
Orthologous residues
SPACA1 (human): A255‑p, SPACA1 (mouse): T265‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  spermatozoa
 Cellular systems studied:  primary cells
 Species studied:  mouse
 Comments:  ambiguous site (within peptides)

S266-p - SPACA1 (mouse)
Orthologous residues
SPACA1 (human): S256‑p, SPACA1 (mouse): S266‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  spermatozoa
 Cellular systems studied:  primary cells
 Species studied:  mouse
 Comments:  ambiguous site (within peptides)

T268-p - SPACA1 (mouse)
Orthologous residues
SPACA1 (human): P258‑p, SPACA1 (mouse): T268‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  spermatozoa
 Cellular systems studied:  primary cells
 Species studied:  mouse

S272-p - SPACA1 (mouse)
Orthologous residues
SPACA1 (human): S262‑p, SPACA1 (mouse): S272‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  spermatozoa
 Cellular systems studied:  primary cells
 Species studied:  mouse

S275-p - SPACA1 (mouse)
Orthologous residues
SPACA1 (human): S265‑p, SPACA1 (mouse): S275‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  spermatozoa
 Cellular systems studied:  primary cells
 Species studied:  mouse

S276-p - SPACA1 (mouse)
Orthologous residues
SPACA1 (human): S266‑p, SPACA1 (mouse): S276‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  spermatozoa
 Cellular systems studied:  primary cells
 Species studied:  mouse

S13-p - SPATA18 (mouse)
Orthologous residues
SPATA18 (human): T13‑p, SPATA18 (mouse): S13‑p, SPATA18 (rat): S13‑p, SPATA18 iso2 (rat): S13‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  spermatozoa
 Cellular systems studied:  primary cells
 Species studied:  mouse

S283-p - SPATA18 (mouse)
Orthologous residues
SPATA18 (human): R285‑p, SPATA18 (mouse): S283‑p, SPATA18 (rat): S303‑p, SPATA18 iso2 (rat): S303‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  spermatozoa
 Cellular systems studied:  primary cells
 Species studied:  mouse
 Comments:  ambiguous site (within proteins)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sperm capacitation increase

S285-p - SPATA18 (mouse)
Orthologous residues
SPATA18 (human): S287‑p, SPATA18 (mouse): S285‑p, SPATA18 (rat): S305‑p, SPATA18 iso2 (rat): S305‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  spermatozoa
 Cellular systems studied:  primary cells
 Species studied:  mouse
 Comments:  ambiguous site (within proteins)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sperm capacitation increase

S526-p - SPATA18 (mouse)
Orthologous residues
SPATA18 (human): S527‑p, SPATA18 (mouse): S526‑p, SPATA18 (rat): S557‑p, SPATA18 iso2 (rat): S545‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  spermatozoa
 Cellular systems studied:  primary cells
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sperm capacitation increase

S528-p - SPATA18 (mouse)
Orthologous residues
SPATA18 (human): S529‑p, SPATA18 (mouse): S528‑p, SPATA18 (rat): S559‑p, SPATA18 iso2 (rat): S547‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  spermatozoa
 Cellular systems studied:  primary cells
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sperm capacitation increase

S72-p - ST6GALNAC2 iso2 (mouse)
Orthologous residues
ST6GALNAC2 (human): , ST6GALNAC2 (mouse): , ST6GALNAC2 iso2 (mouse): S72‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  spermatozoa
 Cellular systems studied:  primary cells
 Species studied:  mouse

S73-p - ST6GALNAC2 iso2 (mouse)
Orthologous residues
ST6GALNAC2 (human): , ST6GALNAC2 (mouse): , ST6GALNAC2 iso2 (mouse): S73‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  spermatozoa
 Cellular systems studied:  primary cells
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sperm capacitation increase

S75-p - ST6GALNAC2 iso2 (mouse)
Orthologous residues
ST6GALNAC2 (human): , ST6GALNAC2 (mouse): , ST6GALNAC2 iso2 (mouse): S75‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  spermatozoa
 Cellular systems studied:  primary cells
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sperm capacitation increase

S77-p - ST6GALNAC2 iso2 (mouse)
Orthologous residues
ST6GALNAC2 (human): , ST6GALNAC2 (mouse): , ST6GALNAC2 iso2 (mouse): S77‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  spermatozoa
 Cellular systems studied:  primary cells
 Species studied:  mouse

S122-p - ST6GALNAC2 iso2 (mouse)
Orthologous residues
ST6GALNAC2 (human): , ST6GALNAC2 (mouse): , ST6GALNAC2 iso2 (mouse): S122‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  spermatozoa
 Cellular systems studied:  primary cells
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sperm capacitation increase

S124-p - ST6GALNAC2 iso2 (mouse)
Orthologous residues
ST6GALNAC2 (human): , ST6GALNAC2 (mouse): , ST6GALNAC2 iso2 (mouse): S124‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  spermatozoa
 Cellular systems studied:  primary cells
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sperm capacitation increase

S126-p - ST6GALNAC2 iso2 (mouse)
Orthologous residues
ST6GALNAC2 (human): , ST6GALNAC2 (mouse): , ST6GALNAC2 iso2 (mouse): S126‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  spermatozoa
 Cellular systems studied:  primary cells
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sperm capacitation increase

S130-p - ST6GALNAC2 iso2 (mouse)
Orthologous residues
ST6GALNAC2 (human): , ST6GALNAC2 (mouse): , ST6GALNAC2 iso2 (mouse): S130‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  spermatozoa
 Cellular systems studied:  primary cells
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sperm capacitation increase

S132-p - ST6GALNAC2 iso2 (mouse)
Orthologous residues
ST6GALNAC2 (human): , ST6GALNAC2 (mouse): , ST6GALNAC2 iso2 (mouse): S132‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  spermatozoa
 Cellular systems studied:  primary cells
 Species studied:  mouse

S271-p - ST6GALNAC2 iso2 (mouse)
Orthologous residues
ST6GALNAC2 (human): , ST6GALNAC2 (mouse): , ST6GALNAC2 iso2 (mouse): S271‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  spermatozoa
 Cellular systems studied:  primary cells
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sperm capacitation increase

S18-p - TCTE3 (mouse)
Orthologous residues
TCTE3 (human): A23‑p, TCTE3 (mouse): S18‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  spermatozoa
 Cellular systems studied:  primary cells
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sperm capacitation increase

S10-p - THEG (mouse)
Orthologous residues
THEG (human): S8‑p, THEG (mouse): S10‑p, THEG (rat): S12‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  spermatozoa
 Cellular systems studied:  primary cells
 Species studied:  mouse

S13-p - THEG (mouse)
Orthologous residues
THEG (human): N11‑p, THEG (mouse): S13‑p, THEG (rat): S15‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  spermatozoa
 Cellular systems studied:  primary cells
 Species studied:  mouse

S57-p - UBA52 (mouse)
Orthologous residues
UBA52 (human): S57‑p, UBA52 (mouse): S57‑p, UBA52 (rat): S57‑p, UBA52 (cow): S57‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  spermatozoa
 Cellular systems studied:  primary cells
 Species studied:  mouse
 Comments:  ambiguous site (between proteins)

S57-p - UBC (mouse)
Orthologous residues
UBC (human): S57‑p, UBC iso18 (human): , UBC (mouse): S57‑p, UBC var1 (mouse): S57‑p, UBC (rat): S57‑p, UBC (cow): S57‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  spermatozoa
 Cellular systems studied:  primary cells
 Species studied:  mouse
 Comments:  ambiguous site (between proteins and within proteins)

S133-p - UBC (mouse)
Orthologous residues
UBC (human): S133‑p, UBC iso18 (human): , UBC (mouse): S133‑p, UBC var1 (mouse): S133‑p, UBC (rat): S133‑p, UBC (cow): S133‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  spermatozoa
 Cellular systems studied:  primary cells
 Species studied:  mouse
 Comments:  ambiguous site (between proteins and within proteins)

S209-p - UBC (mouse)
Orthologous residues
UBC (human): S209‑p, UBC iso18 (human): , UBC (mouse): S209‑p, UBC var1 (mouse): S209‑p, UBC (rat): S209‑p, UBC (cow): S209‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  spermatozoa
 Cellular systems studied:  primary cells
 Species studied:  mouse
 Comments:  ambiguous site (between proteins and within proteins)

S285-p - UBC (mouse)
Orthologous residues
UBC (human): S285‑p, UBC iso18 (human): , UBC (mouse): S285‑p, UBC var1 (mouse): S285‑p, UBC (rat): S285‑p, UBC (cow): S285‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  spermatozoa
 Cellular systems studied:  primary cells
 Species studied:  mouse
 Comments:  ambiguous site (between proteins and within proteins)

S361-p - UBC (mouse)
Orthologous residues
UBC (human): S361‑p, UBC iso18 (human): , UBC (mouse): S361‑p, UBC var1 (mouse): S361‑p, UBC (rat): S361‑p, UBC (cow): S361‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  spermatozoa
 Cellular systems studied:  primary cells
 Species studied:  mouse
 Comments:  ambiguous site (between proteins and within proteins)

S437-p - UBC (mouse)
Orthologous residues
UBC (human): S437‑p, UBC iso18 (human): , UBC (mouse): S437‑p, UBC var1 (mouse): S437‑p, UBC (rat): S437‑p, UBC (cow): S437‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  spermatozoa
 Cellular systems studied:  primary cells
 Species studied:  mouse
 Comments:  ambiguous site (between proteins and within proteins)

S513-p - UBC (mouse)
Orthologous residues
UBC (human): S513‑p, UBC iso18 (human): , UBC (mouse): S513‑p, UBC var1 (mouse): S513‑p, UBC (rat): S513‑p, UBC (cow): S513‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  spermatozoa
 Cellular systems studied:  primary cells
 Species studied:  mouse
 Comments:  ambiguous site (between proteins and within proteins)

S589-p - UBC (mouse)
Orthologous residues
UBC (human): S589‑p, UBC iso18 (human): S57‑p, UBC (mouse): S589‑p, UBC var1 (mouse): S589‑p, UBC (rat): S589‑p, UBC (cow): S589‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  spermatozoa
 Cellular systems studied:  primary cells
 Species studied:  mouse
 Comments:  ambiguous site (between proteins and within proteins)

S665-p - UBC (mouse)
Orthologous residues
UBC (human): S665‑p, UBC iso18 (human): S133‑p, UBC (mouse): S665‑p, UBC var1 (mouse): S665‑p, UBC (rat): S665‑p, UBC (cow): S665‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  spermatozoa
 Cellular systems studied:  primary cells
 Species studied:  mouse
 Comments:  ambiguous site (between proteins and within proteins)

S57-p - ubiquitin (mouse)
Orthologous residues
ubiquitin (human): S57‑p, ubiquitin (mouse): S57‑p, ubiquitin (rat): S57‑p, ubiquitin (cow): S57‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  spermatozoa
 Cellular systems studied:  primary cells
 Species studied:  mouse
 Comments:  ambiguous site (between proteins)

S958-p - WDR64 (mouse)
Orthologous residues
WDR64 (human): S959‑p, WDR64 (mouse): S958‑p, WDR64 (rat): S969‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  spermatozoa
 Cellular systems studied:  primary cells
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sperm capacitation increase

S959-p - WDR64 (mouse)
Orthologous residues
WDR64 (human): S960‑p, WDR64 (mouse): S959‑p, WDR64 (rat): S970‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  spermatozoa
 Cellular systems studied:  primary cells
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sperm capacitation increase


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