Curated Information
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Curated Information Page
PubMed Id: 15073328 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Shi Y, et al. (2004) Direct regulation of CREB transcriptional activity by ATM in response to genotoxic stress. Proc Natl Acad Sci U S A 101, 5898-903 15073328
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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T100-p - CREB (human) ▲
Orthologous residues
CREB (human): T100‑p, CREB (mouse): T100‑p, CREB (rat): T100‑p
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, mutation of modification site
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE ATM (human)
Downstream Regulation
 Effect of modification (function):  inhibition
 Effect of modification (process):  transcription, altered

S111-p - CREB (human) ▲
Orthologous residues
CREB (human): S111‑p, CREB (mouse): S111‑p, CREB (rat): S111‑p
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, mutation of modification site
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Comments:  phosphorylation of this site primes phosphorylation of S121 in response to the stress
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE ATM (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
H2O2 increase
Downstream Regulation
 Effect of modification (function):  inhibition
 Effect of modification (process):  transcription, altered

S121-p - CREB (human) ▲
Orthologous residues
CREB (human): S121‑p, CREB (mouse): S121‑p, CREB (rat): S121‑p
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, mutation of modification site, phospho-antibody, western blotting
 Disease tissue studied:  leukemia, chronic myelogenous leukemia
 Relevant cell lines - cell types - tissues:  293 (epithelial), K562 (erythroid)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE ATM (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (human) genetic knockout/knockin of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
H2O2 increase
ionizing radiation increase
UV increase
aphidicolin increase
deferoxamine increase
Downstream Regulation
 Effect of modification (function):  inhibition
 Effect of modification (process):  transcription, altered

S133-p - CREB (human) ▲
Orthologous residues
CREB (human): S133‑p, CREB (mouse): S133‑p, CREB (rat): S133‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Disease tissue studied:  leukemia, chronic myelogenous leukemia
 Relevant cell lines - cell types - tissues:  K562 (erythroid)
 Cellular systems studied:  cell lines
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing radiation increase
H2O2 increase
Downstream Regulation
 Effect of modification (function):  activation
 Effect of modification (process):  transcription, altered

S121-p - CREB (mouse) ▲
Orthologous residues
CREB (human): S121‑p, CREB (mouse): S121‑p, CREB (rat): S121‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'brain, cerebrum'
 Cellular systems studied:  primary cells
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing radiation increase
wortmannin ionizing radiation inhibit treatment-induced increase


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