|
Orthologous residues
|
|
Chk2 (human): T68‑p, Chk2 iso12 (human): T68‑p, Chk2 (mouse): T77‑p, Chk2 (rat): T76‑p
|
|
Characterization
|
|
Methods used to characterize site in vivo:
electrophoretic mobility shift, phospho-antibody
|
|
Relevant cell lines - cell types - tissues:
293 (epithelial), AT22IJE-T (fibroblast)
|
|
Cellular systems studied:
cell lines
|
|
Species studied:
human
|
|
Enzymes shown to modify site in vitro:
|
|
|
|
Upstream Regulation
|
|
Treatments, proteins and their effect on site modification:
|
|
Treatments
|
Referenced Treatments
|
Manipulated Protein
|
Referenced Protein
|
Effect
|
Notes
|
|
UV
|
|
|
|
increase
|
|
|
ionizing radiation
|
|
|
|
increase
|
|
|
adriamycin
|
|
|
|
increase
|
|
|
camptothecin
|
|
|
|
increase
|
|
|
AP20187
|
|
|
|
increase
|
|
|
|
Downstream Regulation
|
|
Effect of modification (function):
enzymatic activity, induced, intracellular localization, molecular association, regulation
|
|
Modification regulates interactions with:
|
|
Interacting molecule
|
Interacting domains
|
Effect
|
Consequences (function)
|
Consequences (process)
|
Detection assays
|
|
Chk2 (human)
|
|
Induces
|
phosphorylation
|
|
co-immunoprecipitation, electrophoretic visualization
|
|
|
Comments:
phosphorylation of T68 induces Chk2 dissociation from chromatin
|
