Curated Information
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Curated Information Page
PubMed Id: 15980064 
Hurley RL, et al. (2005) The Ca2+/calmodulin-dependent protein kinase kinases are AMP-activated protein kinase kinases. J Biol Chem 280, 29060-6 15980064
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
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T183-p - AMPKA1 (human)
Orthologous residues
AMPKA1 (human): T183‑p, AMPKA1 (mouse): T183‑p, AMPKA1 (rat): T183‑p, AMPKA1 (fruit fly): T184‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody
 Relevant cell lines - cell types - tissues:  A549 (pulmonary), fibroblast-embryo [LKB1 (mouse)], HeLa (cervical) [CAMKK1 (human)]
 Cellular systems studied:  cell lines, primary cells
 Species studied:  human, mouse
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CAMKK2 (human) siRNA inhibition of enzyme, pharmacological inhibitor of upstream enzyme, genetic knockout/knockin of upstream enzyme
KINASE CAMKK1 (human) siRNA inhibition of enzyme, pharmacological inhibitor of upstream enzyme, genetic knockout/knockin of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionomycin increase All three cell types (HeLa, A549, and MEF from LKB-/- mice) lack LKB1.
STO-609 ionomycin inhibit treatment-induced increase All three cell types (HeLa, A549, and MEF from LKB-/- mice) lack LKB1.
2-deoxyglucose increase All three cell types (HeLa, A549, and MEF from LKB-/- mice) lack LKB1.
STO-609 2-deoxyglucose inhibit treatment-induced increase All three cell types (HeLa, A549, and MEF from LKB-/- mice) lack LKB1.
mannitol increase All three cell types (HeLa, A549, and MEF from LKB-/- mice) lack LKB1.
AICAR no change compared to control All three cell types (HeLa, A549, and MEF from LKB-/- mice) lack LKB1.
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced


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