Curated Information
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Curated Information Page
PubMed Id: 12801936 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Dumaz N, Marais R (2003) Protein kinase A blocks Raf-1 activity by stimulating 14-3-3 binding and blocking Raf-1 interaction with Ras. J Biol Chem 278, 29819-23 12801936
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S43-p - c-Raf (human) ▲
Orthologous residues
c‑Raf (human): S43‑p, c‑Raf (mouse): S43‑p, c‑Raf (rat): S43‑p, c‑Raf (chicken): S43‑p
Characterization
 Methods used to characterize site in vivo:  mutation of modification site, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout], COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey, mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF decrease
forskolin, IBMX increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
14-3-3 beta (human) Induces co-immunoprecipitation
HRas (human) Disrupts co-immunoprecipitation

S233-p - c-Raf (human) ▲
Orthologous residues
c‑Raf (human): S233‑p, c‑Raf (mouse): S233‑p, c‑Raf (rat): S233‑p, c‑Raf (chicken): S233‑p
Characterization
 Methods used to characterize site in vivo:  mutation of modification site, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout], COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey, mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF decrease
forskolin, IBMX increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
14-3-3 beta (human) Induces co-immunoprecipitation
HRas (human) Disrupts co-immunoprecipitation

S259-p - c-Raf (human) ▲
Orthologous residues
c‑Raf (human): S259‑p, c‑Raf (mouse): S259‑p, c‑Raf (rat): S259‑p, c‑Raf (chicken): S259‑p
Characterization
 Methods used to characterize site in vivo:  mutation of modification site, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout], COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey, mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF decrease
forskolin, IBMX increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
14-3-3 beta (human) Induces co-immunoprecipitation
HRas (human) Disrupts co-immunoprecipitation


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