Curated Information
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Curated Information Page
PubMed Id: 16000375 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Brown MC, et al. (2005) Src and FAK kinases cooperate to phosphorylate paxillin kinase linker, stimulate its focal adhesion localization, and regulate cell spreading and protrusiveness. Mol Biol Cell 16, 4316-28 16000375
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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Y286-p - GIT2 (chicken)
Orthologous residues
GIT2 (human): Y286‑p, GIT2 iso10 (human): Y288‑p, GIT2 (mouse): Y285‑p, GIT2 iso3 (mouse): Y286‑p, GIT2 iso4 (mouse): Y286‑p, GIT2 iso5 (mouse): Y288‑p, GIT2 (rat): Y286‑p, GIT2 (chicken): Y286‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site
 Relevant cell lines - cell types - tissues:  MEF (fibroblast) [IGF1R (mouse)]
 Cellular systems studied:  primary cultured cells
 Species studied:  mouse
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE FAK (chicken) transfection of inactive enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
fibronectin increase
Downstream Regulation
 Effect of modification (function):  intracellular localization, molecular association, regulation
 Effect of modification (process):  cell adhesion, altered
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
PXN (chicken) Induces cell adhesion, altered co-immunoprecipitation

Y392-p - GIT2 (chicken)
Orthologous residues
GIT2 (human): Y392‑p, GIT2 iso10 (human): Y394‑p, GIT2 (mouse): Y391‑p, GIT2 iso3 (mouse): Y392‑p, GIT2 iso4 (mouse): Y392‑p, GIT2 iso5 (mouse): Y394‑p, GIT2 (rat): Y392‑p, GIT2 (chicken): Y392‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site
 Relevant cell lines - cell types - tissues:  MEF (fibroblast) [IGF1R (mouse)]
 Cellular systems studied:  primary cultured cells
 Species studied:  mouse
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE FAK (chicken) transfection of inactive enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
fibronectin increase
Downstream Regulation
 Effect of modification (function):  intracellular localization, molecular association, regulation
 Effect of modification (process):  cell adhesion, altered
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
PXN (chicken) Induces cell adhesion, altered co-immunoprecipitation

Y592-p - GIT2 (chicken)
Orthologous residues
GIT2 (human): Y592‑p, GIT2 iso10 (human): Y514‑p, GIT2 (mouse): Y541‑p, GIT2 iso3 (mouse): Y512‑p, GIT2 iso4 (mouse): Y592‑p, GIT2 iso5 (mouse): Y514‑p, GIT2 (rat): Y592‑p, GIT2 (chicken): Y592‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site
 Relevant cell lines - cell types - tissues:  MEF (fibroblast) [IGF1R (mouse)]
 Cellular systems studied:  primary cultured cells
 Species studied:  mouse
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE FAK (chicken) transfection of inactive enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
fibronectin increase
Downstream Regulation
 Effect of modification (function):  intracellular localization, molecular association, regulation
 Effect of modification (process):  cell adhesion, altered
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
PXN (chicken) Induces cell adhesion, altered co-immunoprecipitation


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