Curated Information
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Curated Information Page
PubMed Id: 10601308 
Mattingly RR (1999) Phosphorylation of serine 916 of Ras-GRF1 contributes to the activation of exchange factor activity by muscarinic receptors. J Biol Chem 274, 37379-84 10601308
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
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S927-p - RasGRF1 (human)
Orthologous residues
RasGRF1 (human): S927‑p, RasGRF1 iso3 (human): S911‑p, RasGRF1 (mouse): S916‑p, RasGRF1 (rat): S898‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, immunoprecipitation, mutation of modification site, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast), COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey, mouse
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE PKACA (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PKACA (human) pharmacological inhibitor of upstream enzyme, pharmacological activator of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
forskolin, IBMX increase
carbachol increase
Downstream Regulation
 Effect of modification (function):  activity, induced
 Comments:  necessary but not sufficient for RasGRF1 activation


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