Curated Information
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PubMed Id: 9658397 
Rocchi S, et al. (1998) Determination of Gab1 (Grb2-associated binder-1) interaction with insulin receptor-signaling molecules. Mol Endocrinol 12, 914-23 9658397
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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Y447-p - Gab1 (human)
Orthologous residues
Gab1 (human): Y447‑p, Gab1 iso2 (human): Y447‑p, Gab1 (mouse): Y448‑p, Gab1 (rat): Y446‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE InsR (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
SHP-2 (human) Induces co-immunoprecipitation, electrophoretic visualization, yeast two-hybrid
PIK3R1 (human) Induces co-immunoprecipitation, electrophoretic visualization, yeast two-hybrid

Y472-p - Gab1 (human)
Orthologous residues
Gab1 (human): Y472‑p, Gab1 iso2 (human): Y472‑p, Gab1 (mouse): Y473‑p, Gab1 (rat): Y471‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE InsR (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
SHP-2 (human) Induces co-immunoprecipitation, electrophoretic visualization, yeast two-hybrid
PIK3R1 (human) Induces co-immunoprecipitation, electrophoretic visualization, yeast two-hybrid

Y589-p - Gab1 (human)
Orthologous residues
Gab1 (human): Y589‑p, Gab1 iso2 (human): Y619‑p, Gab1 (mouse): Y590‑p, Gab1 (rat): Y588‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE InsR (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
PIK3R1 (human) Induces co-immunoprecipitation, electrophoretic visualization, yeast two-hybrid
SHP-2 (human) Induces co-immunoprecipitation, electrophoretic visualization, yeast two-hybrid

Y627-p - Gab1 (human)
Orthologous residues
Gab1 (human): Y627‑p, Gab1 iso2 (human): Y657‑p, Gab1 (mouse): Y628‑p, Gab1 (rat): Y626‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE InsR (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
PIK3R1 (human) Induces co-immunoprecipitation, electrophoretic visualization, yeast two-hybrid
SHP-2 (human) Induces co-immunoprecipitation, electrophoretic visualization, yeast two-hybrid


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