Curated Information
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Curated Information Page
PubMed Id: 18760948 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
Chuderland D, Konson A, Seger R (2008) Identification and characterization of a general nuclear translocation signal in signaling proteins. Mol Cell 31, 850-61 18760948
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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T202-p - ERK1 (human)
Orthologous residues
ERK1 (human): T202‑p, ERK1 (mouse): T203‑p, ERK1 (rat): T203‑p, ERK1 (hamster): T192‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  CHO (fibroblast), COS (fibroblast), HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  monkey
 Comments:  Phospho-antibody recognizes dually or singly phosphorylated ERK S245 and S247.
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol ester increase ERK2 wild-type
phorbol ester phorbol ester inhibit treatment-induced increase S244-6A mutant

Y204-p - ERK1 (human)
Orthologous residues
ERK1 (human): Y204‑p, ERK1 (mouse): Y205‑p, ERK1 (rat): Y205‑p, ERK1 (hamster): Y194‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  CHO (fibroblast), COS (fibroblast), HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  monkey
 Comments:  Phospho-antibody recognizes dually or singly phosphorylated ERK S245 and S247.
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol ester increase ERK2 wild-type
phorbol ester phorbol ester inhibit treatment-induced increase S244-6A mutant

T185-p - ERK2 (human)
Orthologous residues
ERK2 (human): T185‑p, ERK2 (mouse): T183‑p, ERK2 (rat): T183‑p, ERK2 (chicken): T193‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  CHO (fibroblast), COS (fibroblast), HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  monkey
 Comments:  Phospho-antibody recognizes dually or singly phosphorylated ERK S245 and S247.
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol ester increase ERK2 wild-type
phorbol ester phorbol ester inhibit treatment-induced increase S244-6A mutant

Y187-p - ERK2 (human)
Orthologous residues
ERK2 (human): Y187‑p, ERK2 (mouse): Y185‑p, ERK2 (rat): Y185‑p, ERK2 (chicken): Y195‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  CHO (fibroblast), COS (fibroblast), HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  monkey
 Comments:  Phospho-antibody recognizes dually or singly phosphorylated ERK S245 and S247.
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol ester increase ERK2 wild-type
phorbol ester phorbol ester inhibit treatment-induced increase S244-6A mutant

S246-p - ERK2 (human)
Orthologous residues
ERK2 (human): S246‑p, ERK2 (mouse): S244‑p, ERK2 (rat): S244‑p, ERK2 (chicken): S254‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  CHO (fibroblast), COS (fibroblast), HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  monkey
 Comments:  Phospho-antibody recognizes dually or singly phosphorylated ERK S245 and S247.
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
vanadate increase
phorbol ester increase
EGF increase
Downstream Regulation
 Effect of modification (function):  intracellular localization, molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
IPO7 (human) Induces intracellular localization co-immunoprecipitation
NUP153 (human) Disrupts molecular association, regulation co-immunoprecipitation
 Comments:  nuclear translocation

S248-p - ERK2 (human)
Orthologous residues
ERK2 (human): S248‑p, ERK2 (mouse): S246‑p, ERK2 (rat): S246‑p, ERK2 (chicken): S256‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  CHO (fibroblast), COS (fibroblast), HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  monkey
 Comments:  Phospho-antibody recognizes dually or singly phosphorylated ERK S245 and S247.
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
vanadate increase
phorbol ester increase
EGF increase
Downstream Regulation
 Effect of modification (function):  intracellular localization, molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
NUP153 (human) Disrupts molecular association, regulation co-immunoprecipitation
IPO7 (human) Induces intracellular localization co-immunoprecipitation
 Comments:  nuclear translocation

T386-p - MEK1 (human)
Orthologous residues
MEK1 (human): T386‑p, MEK1 (mouse): T386‑p, MEK1 (rat): T386‑p, MEK1 (rabbit): T386‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  CHO (fibroblast), COS (fibroblast), HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  monkey
 Comments:  Phospho-antibody recognizes dually or singly phosphorylated ERK S245 and S247.
Downstream Regulation
 Effect of modification (function):  intracellular localization
 Comments:  nuclear translocation

T388-p - MEK1 (human)
Orthologous residues
MEK1 (human): T388‑p, MEK1 (mouse): T388‑p, MEK1 (rat): T388‑p, MEK1 (rabbit): T388‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  CHO (fibroblast), COS (fibroblast), HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  monkey
 Comments:  Phospho-antibody recognizes dually or singly phosphorylated ERK S245 and S247.
Downstream Regulation
 Effect of modification (function):  intracellular localization
 Comments:  nuclear translocation

S416-p - Smad3 (human)
Orthologous residues
Smad3 (human): S416‑p, Smad3 (mouse): S416‑p, Smad3 (rat): S416‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  CHO (fibroblast), COS (fibroblast), HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  monkey
 Comments:  Phospho-antibody recognizes dually or singly phosphorylated ERK S245 and S247.
Downstream Regulation
 Effect of modification (function):  intracellular localization
 Comments:  nuclear translocation

S418-p - Smad3 (human)
Orthologous residues
Smad3 (human): S418‑p, Smad3 (mouse): S418‑p, Smad3 (rat): S418‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  CHO (fibroblast), COS (fibroblast), HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  monkey
 Comments:  Phospho-antibody recognizes dually or singly phosphorylated ERK S245 and S247.
Downstream Regulation
 Effect of modification (function):  intracellular localization
 Comments:  nuclear translocation


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