Curated Information
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Curated Information Page
PubMed Id: 16081816 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Houtman JC, et al. (2005) Early phosphorylation kinetics of proteins involved in proximal TCR-mediated signaling pathways. J Immunol 175, 2449-58 16081816
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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Y731-p - Cbl (human) ▲
Orthologous residues
Cbl (human): Y731‑p, Cbl (mouse): Y737‑p, Cbl (rat): Y738‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody
 Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
anti-CD3 increase

Y161-p - LAT (human) ▲
Orthologous residues
LAT (human): Y161‑p, LAT iso2 (human): Y132‑p, LAT (mouse): Y136‑p, LAT (rat): Y136‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody
 Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
anti-CD3 increase
anti-CD4 increase
anti-CD28 increase
anti-CD3, anti-CD4 increase
anti-CD28, anti-CD3 increase

Y220-p - LAT (human) ▲
Orthologous residues
LAT (human): Y220‑p, LAT iso2 (human): Y191‑p, LAT (mouse): Y195‑p, LAT (rat): Y195‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody
 Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
anti-CD3 increase
anti-CD4 increase
anti-CD28 increase
anti-CD3, anti-CD4 increase
anti-CD28, anti-CD3 increase

Y783-p - PLCG1 (human) ▲
Orthologous residues
PLCG1 (human): Y783‑p, PLCG1 iso2 (human): Y783‑p, PLCG1 (mouse): Y783‑p, PLCG1 (rat): Y783‑p, PLCG1 (cow): Y783‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody
 Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
anti-CD3 increase

Y145-p - SLP76 (human) ▲
Orthologous residues
SLP76 (human): Y145‑p, SLP76 (mouse): Y145‑p, SLP76 (rat): Y145‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody
 Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
anti-CD3 increase

Y319-p - ZAP70 (human) ▲
Orthologous residues
ZAP70 (human): Y319‑p, ZAP70 (mouse): Y318‑p, ZAP70 (rat): Y314‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody
 Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
anti-CD3 increase


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