Enomoto A, et al. (2005) Akt/PKB regulates actin organization and cell motility via Girdin/APE. Dev Cell 9, 389-402 16139227

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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S1417-p - girdin (human) ▲

Modsite: INRERQksLtLtPTR SwissProt Entrez-Gene Orthologous residues girdin (human): S1417‑p, girdin (mouse): S1417‑p, girdin (rat): S1397‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody Relevant cell lines - cell types - tissues:  Vero (epithelial) [girdin (human)] Cellular systems studied:  cell lines Enzymes shown to modify site in vitro:  Type Enzyme KINASE Akt1 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE Akt1 (human) transfection of constitutively active enzyme, transfection of wild-type enzyme, inhibition of upstream enzyme, pharmacological inhibitor of upstream enzyme, transfection of dominant-negative enzyme, co-immunoprecipitation, siRNA inhibition of enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes EGF increase LY294002 EGF inhibit treatment-induced increase wortmannin EGF inhibit treatment-induced increase PD98059 EGF no effect upon treatment-induced increase Downstream Regulation Effect of modification (function):  intracellular localization, molecular association, regulation Effect of modification (process):  cell motility, altered, cytoskeletal reorganization Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays ACTB (human) Induces cytoskeletal reorganization, cell motility, altered microscopy-colocalization, yeast two-hybrid