Curated Information
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Curated Information Page
PubMed Id: 16061178 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
López-Bergami P, et al. (2005) RACK1 mediates activation of JNK by protein kinase C [corrected] Mol Cell 19, 309-20 16061178
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S129-p - JNK2 (mouse) ▲
Orthologous residues
JNK2 (human): S129‑p, JNK2 iso2 (human): S129‑p, JNK2 iso3 (human): S129‑p, JNK2 (mouse): S129‑p, JNK2 (rat): S129‑p
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, mutation of modification site, phospho-antibody, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  293T (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE PKCB iso2 (human)
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced

T183-p - JNK2 (mouse) ▲
Orthologous residues
JNK2 (human): T183‑p, JNK2 iso2 (human): T183‑p, JNK2 iso3 (human): T183‑p, JNK2 (mouse): T183‑p, JNK2 (rat): T183‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, phospho-antibody
 Relevant cell lines - cell types - tissues:  U2OS (bone cell) [GR (human)]
 Cellular systems studied:  cell lines
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE MKK4 (human) antisense inhibition of upstream enzyme
KINASE MKK7 (human) antisense inhibition of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
UV increase
phorbol ester increase
TNF increase
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced

Y185-p - JNK2 (mouse) ▲
Orthologous residues
JNK2 (human): Y185‑p, JNK2 iso2 (human): Y185‑p, JNK2 iso3 (human): Y185‑p, JNK2 (mouse): Y185‑p, JNK2 (rat): Y185‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, phospho-antibody
 Relevant cell lines - cell types - tissues:  U2OS (bone cell) [GR (human)]
 Cellular systems studied:  cell lines
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE MKK4 (human) antisense inhibition of upstream enzyme
KINASE MKK7 (human) antisense inhibition of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
UV increase
phorbol ester increase
TNF increase
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced


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