Kim I, et al. (2009) Chemical biology investigation of cell death pathways activated by endoplasmic reticulum stress reveals cytoprotective modulators of ASK1. J Biol Chem 284, 1593-603 19004820

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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S83-p - ASK1 (human) ▲

Modsite: ATRGRGssVGGGSrR SwissProt Entrez-Gene Orthologous residues ASK1 (human): S83‑p, ASK1 iso5 (human): S163‑p, ASK1 (mouse): S90‑p, ASK1 (rat): S90‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, phospho-antibody, western blotting Disease tissue studied:  brain cancer, glioblastoma, glioma, ovarian cancer Relevant cell lines - cell types - tissues:  HEK293T (epithelial), OVCAR5 (ovarian), SNB-19 (glial) Cellular systems studied:  cell lines Species studied:  human Comments:  CSM14.1 rat straital neuroprogenitor cell line Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes benzodiazepinones increase

T838-p - ASK1 (human) ▲

Modsite: GINPCTEtFTGtLQY SwissProt Entrez-Gene Orthologous residues ASK1 (human): T838‑p, ASK1 iso5 (human): T918‑p, ASK1 (mouse): T845‑p, ASK1 (rat): T844‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, phospho-antibody, western blotting Disease tissue studied:  brain cancer, glioblastoma, glioma, ovarian cancer Relevant cell lines - cell types - tissues:  HEK293T (epithelial), OVCAR5 (ovarian), SNB-19 (glial) Cellular systems studied:  cell lines Species studied:  human Comments:  CSM14.1 rat straital neuroprogenitor cell line Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes benzodiazepinones increase

S966-p - ASK1 (human) ▲

Modsite: NEYLRsIsLPVPVLV SwissProt Entrez-Gene Orthologous residues ASK1 (human): S966‑p, ASK1 iso5 (human): S1046‑p, ASK1 (mouse): S973‑p, ASK1 (rat): S972‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, phospho-antibody, western blotting Disease tissue studied:  brain cancer, glioblastoma, glioma, ovarian cancer Relevant cell lines - cell types - tissues:  HEK293T (epithelial), OVCAR5 (ovarian), SNB-19 (glial) Cellular systems studied:  cell lines Species studied:  human Comments:  CSM14.1 rat straital neuroprogenitor cell line Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes benzodiazepinones increase benzodiazepinones increase thapsigargin benzodiazepinones augment treatment-induced increase thapsigargin no change compared to control Downstream Regulation Effect of modification (function):  enzymatic activity, inhibited, molecular association, regulation Effect of modification (process):  apoptosis, induced Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays 14-3-3 beta (human) Induces co-immunoprecipitation Comments:  induced by CID-2891837

S52-p - eIF2-alpha (rat) ▲

Modsite: MILLSELsRRRIRSI SwissProt Entrez-Gene Orthologous residues eIF2‑alpha (human): S52‑p, eIF2‑alpha (mouse): S52‑p, eIF2‑alpha (rat): S52‑p, eIF2‑alpha (rabbit): S51‑p, eIF2‑alpha (fruit fly): S51‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, phospho-antibody, western blotting Disease tissue studied:  brain cancer, glioblastoma, glioma, ovarian cancer Relevant cell lines - cell types - tissues:  HEK293T (epithelial), OVCAR5 (ovarian), SNB-19 (glial) Cellular systems studied:  cell lines Species studied:  human Comments:  CSM14.1 rat straital neuroprogenitor cell line

S73-p - Jun (rat) ▲

Modsite: VGLLKLAsPELERLI SwissProt Entrez-Gene Orthologous residues Jun (human): S73‑p, Jun (mouse): S73‑p, Jun (rat): S73‑p, Jun (cow): S73‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, phospho-antibody, western blotting Disease tissue studied:  brain cancer, glioblastoma, glioma, ovarian cancer Relevant cell lines - cell types - tissues:  HEK293T (epithelial), OVCAR5 (ovarian), SNB-19 (glial) Cellular systems studied:  cell lines Species studied:  human Comments:  CSM14.1 rat straital neuroprogenitor cell line Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes benzodiazepinones no change compared to control

T180-p - P38A (rat) ▲

Modsite: RHTDDEMtGyVATRW SwissProt Entrez-Gene Orthologous residues P38A (human): T180‑p, P38A iso2 (human): T180‑p, P38A (mouse): T180‑p, P38A iso3 (mouse): T180‑p, P38A (rat): T180‑p, P38A (salmonid): T181‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, phospho-antibody, western blotting Disease tissue studied:  brain cancer, glioblastoma, glioma, ovarian cancer Relevant cell lines - cell types - tissues:  HEK293T (epithelial), OVCAR5 (ovarian), SNB-19 (glial) Cellular systems studied:  cell lines Species studied:  human Comments:  CSM14.1 rat straital neuroprogenitor cell line Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes benzodiazepinones no change compared to control

Y182-p - P38A (rat) ▲

Modsite: TDDEMtGyVATRWYR SwissProt Entrez-Gene Orthologous residues P38A (human): Y182‑p, P38A iso2 (human): Y182‑p, P38A (mouse): Y182‑p, P38A iso3 (mouse): Y182‑p, P38A (rat): Y182‑p, P38A (salmonid): Y183‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, phospho-antibody, western blotting Disease tissue studied:  brain cancer, glioblastoma, glioma, ovarian cancer Relevant cell lines - cell types - tissues:  HEK293T (epithelial), OVCAR5 (ovarian), SNB-19 (glial) Cellular systems studied:  cell lines Species studied:  human Comments:  CSM14.1 rat straital neuroprogenitor cell line Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes benzodiazepinones no change compared to control