Curated Information
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Curated Information Page
PubMed Id: 16129678 
Weigert C, et al. (2005) The phosphorylation of Ser318 of insulin receptor substrate 1 is not per se inhibitory in skeletal muscle cells but is necessary to trigger the attenuation of the insulin-stimulated signal. J Biol Chem 280, 37393-9 16129678
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S323-p - IRS1 (human)
Orthologous residues
IRS1 (human): S323‑p, IRS1 (mouse): S318‑p, IRS1 (rat): S318‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, phospho-antibody
 Relevant cell lines - cell types - tissues:  'muscle, skeletal'
 Cellular systems studied:  tissue
 Species studied:  human
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PKCZ (human) competition with site-specific peptide
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IGF-1 increase
phorbol ester increase
insulin increase
LY294002 insulin inhibit treatment-induced increase
wortmannin insulin inhibit treatment-induced increase
peptide antagonist insulin inhibit treatment-induced increase

T410-p - PKCZ (human)
Orthologous residues
PKCZ (human): T410‑p, PKCZ (mouse): T410‑p, PKCZ (rat): T410‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody
 Relevant cell lines - cell types - tissues:  'muscle, skeletal'
 Cellular systems studied:  tissue
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation

S318-p - IRS1 (mouse)
Orthologous residues
IRS1 (human): S323‑p, IRS1 (mouse): S318‑p, IRS1 (rat): S318‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody
 Relevant cell lines - cell types - tissues:  'muscle, skeletal'
 Cellular systems studied:  tissue
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase


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