Curated Information
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PubMed Id: 11274179 
Zang M, et al. (2001) Microtubule integrity regulates Pak leading to Ras-independent activation of Raf-1. insights into mechanisms of Raf-1 activation. J Biol Chem 276, 25157-65 11274179
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S473-p - Akt1 (human)
Orthologous residues
Akt1 (human): S473‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody
 Relevant cell lines - cell types - tissues:  293T (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole no change compared to control
phorbol ester no change compared to control

S338-p - RAF1 (human)
Orthologous residues
RAF1 (human): S338‑p, RAF1 (mouse): S338‑p, RAF1 (rat): S338‑p, RAF1 (chicken): S338‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, phospho-antibody
 Relevant cell lines - cell types - tissues:  293T (epithelial), COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human, monkey
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE PAK1 (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase
phorbol ester increase
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced

S339-p - RAF1 (human)
Orthologous residues
RAF1 (human): S339‑p, RAF1 (mouse): S339‑p, RAF1 (rat): S339‑p, RAF1 (chicken): S339‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site
 Relevant cell lines - cell types - tissues:  COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced

Y340-p - RAF1 (human)
Orthologous residues
RAF1 (human): Y340‑p, RAF1 (mouse): Y340‑p, RAF1 (rat): Y340‑p, RAF1 (chicken): Y340‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site
 Relevant cell lines - cell types - tissues:  COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced

Y341-p - RAF1 (human)
Orthologous residues
RAF1 (human): Y341‑p, RAF1 (mouse): Y341‑p, RAF1 (rat): Y341‑p, RAF1 (chicken): Y341‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site
 Relevant cell lines - cell types - tissues:  COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced


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