Curated Information
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Curated Information Page
PubMed Id: 11285227 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
Bech-Otschir D, et al. (2001) COP9 signalosome-specific phosphorylation targets p53 to degradation by the ubiquitin system. EMBO J 20, 1630-9 11285227
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
Download Sites

S149-p - p53 (human) ▲
Orthologous residues
p53 (human): S149‑p, p53 (mouse): A143‑p, p53 iso2 (mouse): A146‑p, p53 (rat): S147‑p, p53 (rabbit): S146‑p, p53 (monkey): S149‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
 Disease tissue studied:  breast cancer
 Relevant cell lines - cell types - tissues:  HeLa (cervical), MCF-7 (breast cell)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE CK2A1 (human)

T150-p - p53 (human) ▲
Orthologous residues
p53 (human): T150‑p, p53 (mouse): T144‑p, p53 iso2 (mouse): T147‑p, p53 (rat): T148‑p, p53 (rabbit): T147‑p, p53 (monkey): T150‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
 Disease tissue studied:  breast cancer
 Relevant cell lines - cell types - tissues:  HeLa (cervical), MCF-7 (breast cell)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE CK2A1 (human)

T155-p - p53 (human) ▲
Orthologous residues
p53 (human): T155‑p, p53 (mouse): S149‑p, p53 iso2 (mouse): S152‑p, p53 (rat): T153‑p, p53 (rabbit): T152‑p, p53 (monkey): S155‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
 Disease tissue studied:  breast cancer
 Relevant cell lines - cell types - tissues:  HeLa (cervical), MCF-7 (breast cell)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE CK2A1 (human)
Downstream Regulation
 Effect of modification (function):  protein degradation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
MDM2 (human) Induces pull-down assay, in vitro


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