Curated Information
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Curated Information Page
PubMed Id: 14657031 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Kraft C, et al. (2003) Mitotic regulation of the human anaphase-promoting complex by phosphorylation. EMBO J 22, 6598-609 14657031
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
Download Sites

S202-p - APC1 (human)
Orthologous residues
APC1 (human): S202‑p, APC1 (mouse): L202‑p, APC1 (rat): L202‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase

S286-p - APC1 (human)
Orthologous residues
APC1 (human): S286‑p, APC1 (mouse): P286‑p, APC1 (rat): P286‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase

T291-p - APC1 (human)
Orthologous residues
APC1 (human): T291‑p, APC1 (mouse): T291‑p, APC1 (rat): T291‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE PLK1 (human)
KINASE CDK1 (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase

S355-p - APC1 (human)
Orthologous residues
APC1 (human): S355‑p, APC1 (mouse): S355‑p, APC1 (rat): S355‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, phospho-antibody
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE PLK1 (human)
KINASE CDK1 (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase
Downstream Regulation
 Effect of modification (function):  activity, induced

S373-p - APC1 (human)
Orthologous residues
APC1 (human): S373‑p, APC1 (mouse): S373‑p, APC1 (rat): S373‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE PLK1 (human)
KINASE CDK1 (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase

S377-p - APC1 (human)
Orthologous residues
APC1 (human): S377‑p, APC1 (mouse): S377‑p, APC1 (rat): S377‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE PLK1 (human)
KINASE CDK1 (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase

T520-p - APC1 (human)
Orthologous residues
APC1 (human): T520‑p, APC1 (mouse): T520‑p, APC1 (rat): T520‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE PLK1 (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
hydroxyurea increase

T530-p - APC1 (human)
Orthologous residues
APC1 (human): T530‑p, APC1 (mouse): T530‑p, APC1 (rat): T530‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)
KINASE PLK1 (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
hydroxyurea increase

T537-p - APC1 (human)
Orthologous residues
APC1 (human): T537‑p, APC1 (mouse): T537‑p, APC1 (rat): T537‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase

S547-p - APC1 (human)
Orthologous residues
APC1 (human): S547‑p, APC1 (mouse): S547‑p, APC1 (rat): S547‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)
KINASE PLK1 (human)

S555-p - APC1 (human)
Orthologous residues
APC1 (human): S555‑p, APC1 (mouse): S555‑p, APC1 (rat): S555‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)

S569-p - APC1 (human)
Orthologous residues
APC1 (human): S569‑p, APC1 (mouse): S569‑p, APC1 (rat): S569‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
hydroxyurea increase

Y571-p - APC1 (human)
Orthologous residues
APC1 (human): Y571‑p, APC1 (mouse): Y571‑p, APC1 (rat): Y571‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase

S688-p - APC1 (human)
Orthologous residues
APC1 (human): S688‑p, APC1 (mouse): S688‑p, APC1 (rat): S688‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)
KINASE PLK1 (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase

T701-p - APC1 (human)
Orthologous residues
APC1 (human): T701‑p, APC1 (mouse): T701‑p, APC1 (rat): T701‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE PLK1 (human)
KINASE CDK1 (human)

S731-p - APC1 (human)
Orthologous residues
APC1 (human): S731‑p, APC1 (mouse): T731‑p, APC1 (rat): N731‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
hydroxyurea increase

S314-p - APC2 (human)
Orthologous residues
APC2 (human): S314‑p, APC2 (mouse): S329‑p, APC2 (rat): S31‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase

S534-p - APC2 (human)
Orthologous residues
APC2 (human): S534‑p, APC2 (mouse): S549‑p, APC2 (rat): S251‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase

Y469-p - APC4 (human)
Orthologous residues
APC4 (human): Y469‑p, APC4 (mouse): Y469‑p, APC4 (rat): Y469‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase

S779-p - APC4 (human)
Orthologous residues
APC4 (human): S779‑p, APC4 (mouse): S779‑p, APC4 (rat): S779‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE PLK1 (human)
KINASE CDK1 (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase

S195-p - APC5 (human)
Orthologous residues
APC5 (human): S195‑p, APC5 (mouse): S180‑p, APC5 (rat): S180‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase
hydroxyurea increase

S51-p - APC7 (human)
Orthologous residues
APC7 (human): S51‑p, APC7 (mouse): S17‑p, APC7 (rat): S17‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE PLK1 (human)

S57-p - APC7 (human)
Orthologous residues
APC7 (human): S57‑p, APC7 (mouse): S23‑p, APC7 (rat): S23‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE PLK1 (human)

S64-p - APC7 (human)
Orthologous residues
APC7 (human): S64‑p, APC7 (mouse): S30‑p, APC7 (rat): S30‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE PLK1 (human)

S112-p - Cdc16 (human)
Orthologous residues
Cdc16 (human): S112‑p, Cdc16 (mouse): N112‑p, Cdc16 (rat): S112‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)
KINASE PLK1 (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase

S490-p - Cdc16 (human)
Orthologous residues
Cdc16 (human): S490‑p, Cdc16 (mouse): S490‑p, Cdc16 (rat): S490‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase

S560-p - Cdc16 (human)
Orthologous residues
Cdc16 (human): S560‑p, Cdc16 (mouse): S560‑p, Cdc16 (rat): S560‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, phospho-antibody
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase

T581-p - Cdc16 (human)
Orthologous residues
Cdc16 (human): T581‑p, Cdc16 (mouse): A581‑p, Cdc16 (rat): A581‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase

S595-p - Cdc16 (human)
Orthologous residues
Cdc16 (human): S595‑p, Cdc16 (mouse): N595‑p, Cdc16 (rat): S595‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase

T599-p - Cdc16 (human)
Orthologous residues
Cdc16 (human): T599‑p, Cdc16 (mouse): T599‑p, Cdc16 (rat): T599‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase

T70-p - Cdc20 (human)
Orthologous residues
Cdc20 (human): T70‑p, Cdc20 (mouse): T70‑p, Cdc20 (rat): T70‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase

T106-p - Cdc20 (human)
Orthologous residues
Cdc20 (human): T106‑p, Cdc20 (mouse): T106‑p, Cdc20 (rat): T106‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase

Y273-p - Cdc23 (human)
Orthologous residues
Cdc23 (human): Y273‑p, Cdc23 (mouse): Y273‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase

T562-p - Cdc23 (human)
Orthologous residues
Cdc23 (human): T562‑p, Cdc23 (mouse): T562‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, phospho-antibody
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE PLK1 (human)
KINASE CDK1 (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase

T565-p - Cdc23 (human)
Orthologous residues
Cdc23 (human): T565‑p, Cdc23 (mouse): S565‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE PLK1 (human)
KINASE CDK1 (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase

T205-p - Cdc27 (human)
Orthologous residues
Cdc27 (human): T205‑p, Cdc27 iso2 (human): T205‑p, Cdc27 (mouse): T205‑p, Cdc27 iso2 (mouse): T186‑p, Cdc27 (rat): T205‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE PLK1 (human)
KINASE CDK1 (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase

T209-p - Cdc27 (human)
Orthologous residues
Cdc27 (human): T209‑p, Cdc27 iso2 (human): T209‑p, Cdc27 (mouse): T209‑p, Cdc27 iso2 (mouse): T190‑p, Cdc27 (rat): T209‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE PLK1 (human)
KINASE CDK1 (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase

S222-p - Cdc27 (human)
Orthologous residues
Cdc27 (human): S222‑p, Cdc27 iso2 (human): S222‑p, Cdc27 (mouse): S222‑p, Cdc27 iso2 (mouse): S203‑p, Cdc27 (rat): S222‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)

T244-p - Cdc27 (human)
Orthologous residues
Cdc27 (human): T244‑p, Cdc27 iso2 (human): T244‑p, Cdc27 (mouse): N244‑p, Cdc27 iso2 (mouse): N225‑p, Cdc27 (rat): N244‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase

T289-p - Cdc27 (human)
Orthologous residues
Cdc27 (human): T289‑p, Cdc27 iso2 (human): T289‑p, Cdc27 (mouse): T289‑p, Cdc27 iso2 (mouse): T270‑p, Cdc27 (rat): T289‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)

S291-p - Cdc27 (human)
Orthologous residues
Cdc27 (human): S291‑p, Cdc27 iso2 (human): S291‑p, Cdc27 (mouse): S291‑p, Cdc27 iso2 (mouse): S272‑p, Cdc27 (rat): S291‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase

S296-p - Cdc27 (human)
Orthologous residues
Cdc27 (human): S296‑p, Cdc27 iso2 (human): S296‑p, Cdc27 (mouse): S296‑p, Cdc27 iso2 (mouse): S277‑p, Cdc27 (rat): S296‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human

S312-p - Cdc27 (human)
Orthologous residues
Cdc27 (human): S312‑p, Cdc27 iso2 (human): S312‑p, Cdc27 (mouse): P312‑p, Cdc27 iso2 (mouse): P293‑p, Cdc27 (rat): S312‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)

T313-p - Cdc27 (human)
Orthologous residues
Cdc27 (human): T313‑p, Cdc27 iso2 (human): T313‑p, Cdc27 (mouse): T313‑p, Cdc27 iso2 (mouse): N294‑p, Cdc27 (rat): T313‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase

T329-p - Cdc27 (human)
Orthologous residues
Cdc27 (human): T329‑p, Cdc27 iso2 (human): T335‑p, Cdc27 (mouse): T329‑p, Cdc27 iso2 (mouse): T310‑p, Cdc27 (rat): A329‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)

T343-p - Cdc27 (human)
Orthologous residues
Cdc27 (human): T343‑p, Cdc27 iso2 (human): T349‑p, Cdc27 (mouse): T343‑p, Cdc27 iso2 (mouse): T324‑p, Cdc27 (rat): T343‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)

S426-p - Cdc27 (human)
Orthologous residues
Cdc27 (human): S426‑p, Cdc27 iso2 (human): S432‑p, Cdc27 (mouse): S427‑p, Cdc27 iso2 (mouse): , Cdc27 (rat): S427‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, phospho-antibody
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)
KINASE PLK1 (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase

T430-p - Cdc27 (human)
Orthologous residues
Cdc27 (human): T430‑p, Cdc27 iso2 (human): T436‑p, Cdc27 (mouse): T431‑p, Cdc27 iso2 (mouse): , Cdc27 (rat): T431‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)
KINASE PLK1 (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase

S434-p - Cdc27 (human)
Orthologous residues
Cdc27 (human): S434‑p, Cdc27 iso2 (human): S440‑p, Cdc27 (mouse): S435‑p, Cdc27 iso2 (mouse): , Cdc27 (rat): S435‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)
KINASE PLK1 (human)

S435-p - Cdc27 (human)
Orthologous residues
Cdc27 (human): S435‑p, Cdc27 iso2 (human): S441‑p, Cdc27 (mouse): S436‑p, Cdc27 iso2 (mouse): , Cdc27 (rat): S436‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE PLK1 (human)
KINASE CDK1 (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase

T446-p - Cdc27 (human)
Orthologous residues
Cdc27 (human): T446‑p, Cdc27 iso2 (human): T452‑p, Cdc27 (mouse): T447‑p, Cdc27 iso2 (mouse): , Cdc27 (rat): T447‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, phospho-antibody
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)
KINASE PLK1 (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase


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