Curated Information
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Curated Information Page
PubMed Id: 15568999 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
McCoy CE, et al. (2005) MSK1 activity is controlled by multiple phosphorylation sites. Biochem J 387, 507-17 15568999
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
Download Sites

S212-p - MSK1 (human)
Orthologous residues
MSK1 (human): S212‑p, MSK1 (mouse): S211‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mass spectrometry, peptide sequencing, phospho-antibody, phosphopeptide mapping, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE MSK1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE MSK1 (human) transfection of inactive enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol ester increase
PD184352 phorbol ester inhibit treatment-induced increase
UV increase
SB203580 UV inhibit treatment-induced increase
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced

S360-p - MSK1 (human)
Orthologous residues
MSK1 (human): S360‑p, MSK1 (mouse): S359‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mass spectrometry, peptide sequencing, phospho-antibody, phosphopeptide mapping, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ERK2 (human) pharmacological inhibitor of upstream enzyme
KINASE P38A (human) pharmacological inhibitor of upstream enzyme
KINASE ERK1 (human) pharmacological inhibitor of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol ester increase
PD184352 phorbol ester inhibit treatment-induced increase
UV increase
SB203580 UV inhibit treatment-induced increase

S376-p - MSK1 (human)
Orthologous residues
MSK1 (human): S376‑p, MSK1 (mouse): S375‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mass spectrometry, peptide sequencing, phospho-antibody, phosphopeptide mapping, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE MSK1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE MSK1 (human) transfection of inactive enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol ester increase
PD184352 phorbol ester inhibit treatment-induced increase
UV increase
SB203580 UV inhibit treatment-induced increase
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced

S381-p - MSK1 (human)
Orthologous residues
MSK1 (human): S381‑p, MSK1 (mouse): S380‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mass spectrometry, mutation of modification site, peptide sequencing, phospho-antibody, phosphopeptide mapping, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE MSK1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE MSK1 (human) transfection of inactive enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol ester increase
PD184352 phorbol ester inhibit treatment-induced increase
UV increase
SB203580 UV inhibit treatment-induced increase

T581-p - MSK1 (human)
Orthologous residues
MSK1 (human): T581‑p, MSK1 (mouse): T645‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mass spectrometry, mutation of modification site, peptide sequencing, phospho-antibody, phosphopeptide mapping, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE P38A (human) pharmacological inhibitor of upstream enzyme
KINASE ERK1 (human) pharmacological inhibitor of upstream enzyme
KINASE ERK2 (human) pharmacological inhibitor of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol ester increase
PD184352 phorbol ester inhibit treatment-induced increase
UV increase
SB203580 UV inhibit treatment-induced increase
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced

S750-p - MSK1 (human)
Orthologous residues
MSK1 (human): S750‑p, MSK1 (mouse): S814‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mass spectrometry, peptide sequencing, phospho-antibody, phosphopeptide mapping, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE MSK1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE MSK1 (human) transfection of inactive enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol ester increase
PD184352 phorbol ester inhibit treatment-induced increase
UV increase
SB203580 UV inhibit treatment-induced increase

S752-p - MSK1 (human)
Orthologous residues
MSK1 (human): S752‑p, MSK1 (mouse): S816‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mass spectrometry, peptide sequencing, phospho-antibody, phosphopeptide mapping, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE MSK1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE MSK1 (human) transfection of inactive enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol ester increase
PD184352 phorbol ester inhibit treatment-induced increase
UV increase
SB203580 UV inhibit treatment-induced increase

S758-p - MSK1 (human)
Orthologous residues
MSK1 (human): S758‑p, MSK1 (mouse): S822‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mass spectrometry, peptide sequencing, phospho-antibody, phosphopeptide mapping, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE MSK1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE MSK1 (human) transfection of inactive enzyme


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