Curated Information
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Curated Information Page
PubMed Id: 15448698 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Zhou BP, et al. (2004) Dual regulation of Snail by GSK-3beta-mediated phosphorylation in control of epithelial-mesenchymal transition. Nat Cell Biol 6, 931-40 15448698
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S473-p - Akt1 (human)
Orthologous residues
Akt1 (human): S473‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody
 Disease tissue studied:  breast cancer
 Relevant cell lines - cell types - tissues:  293 (epithelial), MCF-7 (breast cell)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IGF-1 increase Snail-MCF-7, Snail HEK293
EGF increase
IGF-1 EGF increase
IGF-1 increase Snail-MCF-7, Snail-HEK 293
wortmannin IGF-1 inhibit treatment-induced increase
PD98059 IGF-1 no effect upon treatment-induced increase
U0126 IGF-1 augment treatment-induced increase

T202-p - ERK1 (human)
Orthologous residues
ERK1 (human): T202‑p, ERK1 (mouse): T203‑p, ERK1 (rat): T203‑p, ERK1 (hamster): T192‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody
 Disease tissue studied:  breast cancer
 Relevant cell lines - cell types - tissues:  293 (epithelial), MCF-7 (breast cell)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IGF-1 increase Snail-MCF-7, Snail HEK293
EGF increase
IGF-1 EGF increase
IGF-1 no change compared to control
wortmannin no change compared to control
PD98059 decrease
U0126 decrease

Y204-p - ERK1 (human)
Orthologous residues
ERK1 (human): Y204‑p, ERK1 (mouse): Y205‑p, ERK1 (rat): Y205‑p, ERK1 (hamster): Y194‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody
 Disease tissue studied:  breast cancer
 Relevant cell lines - cell types - tissues:  293 (epithelial), MCF-7 (breast cell)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IGF-1 increase Snail-MCF-7, Snail HEK293
EGF increase
IGF-1 EGF increase
IGF-1 no change compared to control
wortmannin no change compared to control
PD98059 decrease
U0126 decrease

T185-p - ERK2 (human)
Orthologous residues
ERK2 (human): T185‑p, ERK2 (mouse): T183‑p, ERK2 (rat): T183‑p, ERK2 (chicken): T193‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody
 Disease tissue studied:  breast cancer
 Relevant cell lines - cell types - tissues:  293 (epithelial), MCF-7 (breast cell)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IGF-1 increase Snail-MCF-7, Snail HEK293
EGF increase
IGF-1 EGF increase
IGF-1 no change compared to control
wortmannin no change compared to control
PD98059 decrease
U0126 decrease

Y187-p - ERK2 (human)
Orthologous residues
ERK2 (human): Y187‑p, ERK2 (mouse): Y185‑p, ERK2 (rat): Y185‑p, ERK2 (chicken): Y195‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody
 Disease tissue studied:  breast cancer
 Relevant cell lines - cell types - tissues:  293 (epithelial), MCF-7 (breast cell)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IGF-1 increase Snail-MCF-7, Snail HEK293
EGF increase
IGF-1 EGF increase
IGF-1 no change compared to control
wortmannin no change compared to control
PD98059 decrease
U0126 decrease

S9-p - GSK3B (human)
Orthologous residues
GSK3B (human): S9‑p, GSK3B iso2 (human): S9‑p, GSK3B (mouse): S9‑p, GSK3B (rat): S9‑p, GSK3B (rabbit): S3‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody
 Disease tissue studied:  breast cancer
 Relevant cell lines - cell types - tissues:  293 (epithelial), MCF-7 (breast cell)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IGF-1 increase Snail-MCF-7, Snail HEK293
EGF increase
IGF-1 EGF increase
IGF-1 increase
wortmannin IGF-1 inhibit treatment-induced increase
PD98059 IGF-1 inhibit treatment-induced increase
U0126 IGF-1 inhibit treatment-induced increase
MG132 IGF-1 no effect upon treatment-induced increase

S96-p - Snail1 (human)
Orthologous residues
Snail1 (human): S96‑p, Snail1 (mouse): S96‑p
Characterization
 Methods used to characterize site in vivo microscopy-colocalization with upstream kinase, mutation of modification site, phospho-antibody, western blotting
 Disease tissue studied:  breast cancer
 Relevant cell lines - cell types - tissues:  293 (epithelial), MCF-7 (breast cell)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE GSK3B (human) transfection of constitutively active enzyme, pharmacological inhibitor of upstream enzyme, co-immunoprecipitation, mutation in upstream enzyme recognition motif, transfection of inactive enzyme, transfection of wild-type enzyme
Downstream Regulation
 Effect of modification (function):  protein degradation, ubiquitination
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
FBW1B (human) Induces protein degradation, ubiquitination co-immunoprecipitation

S100-p - Snail1 (human)
Orthologous residues
Snail1 (human): S100‑p, Snail1 (mouse): S100‑p
Characterization
 Methods used to characterize site in vivo microscopy-colocalization with upstream kinase, mutation of modification site, phospho-antibody, western blotting
 Disease tissue studied:  breast cancer
 Relevant cell lines - cell types - tissues:  293 (epithelial), MCF-7 (breast cell)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE GSK3B (human) transfection of constitutively active enzyme, pharmacological inhibitor of upstream enzyme, co-immunoprecipitation, mutation in upstream enzyme recognition motif, transfection of inactive enzyme, transfection of wild-type enzyme
Downstream Regulation
 Effect of modification (function):  protein degradation, ubiquitination
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
FBW1B (human) Induces protein degradation, ubiquitination co-immunoprecipitation

S107-p - Snail1 (human)
Orthologous residues
Snail1 (human): S107‑p, Snail1 (mouse): S107‑p
Characterization
 Methods used to characterize site in vivo microscopy-colocalization with upstream kinase, mutation of modification site, phospho-antibody, western blotting
 Disease tissue studied:  breast cancer
 Relevant cell lines - cell types - tissues:  293 (epithelial), MCF-7 (breast cell)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE GSK3B (human) transfection of constitutively active enzyme, pharmacological inhibitor of upstream enzyme, co-immunoprecipitation, mutation in upstream enzyme recognition motif, transfection of inactive enzyme, transfection of wild-type enzyme
Downstream Regulation
 Effect of modification (function):  intracellular localization

S111-p - Snail1 (human)
Orthologous residues
Snail1 (human): S111‑p, Snail1 (mouse): S111‑p
Characterization
 Methods used to characterize site in vivo microscopy-colocalization with upstream kinase, mutation of modification site, phospho-antibody, western blotting
 Disease tissue studied:  breast cancer
 Relevant cell lines - cell types - tissues:  293 (epithelial), MCF-7 (breast cell)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE GSK3B (human) transfection of constitutively active enzyme, pharmacological inhibitor of upstream enzyme, co-immunoprecipitation, mutation in upstream enzyme recognition motif, transfection of inactive enzyme, transfection of wild-type enzyme
Downstream Regulation
 Effect of modification (function):  intracellular localization

S115-p - Snail1 (human)
Orthologous residues
Snail1 (human): S115‑p, Snail1 (mouse): S115‑p
Characterization
 Methods used to characterize site in vivo microscopy-colocalization with upstream kinase, mutation of modification site, phospho-antibody, western blotting
 Disease tissue studied:  breast cancer
 Relevant cell lines - cell types - tissues:  293 (epithelial), MCF-7 (breast cell)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE GSK3B (human) transfection of constitutively active enzyme, pharmacological inhibitor of upstream enzyme, co-immunoprecipitation, mutation in upstream enzyme recognition motif, transfection of inactive enzyme, transfection of wild-type enzyme
Downstream Regulation
 Effect of modification (function):  intracellular localization

S119-p - Snail1 (human)
Orthologous residues
Snail1 (human): S119‑p, Snail1 (mouse): S119‑p
Characterization
 Methods used to characterize site in vivo microscopy-colocalization with upstream kinase, mutation of modification site, phospho-antibody, western blotting
 Disease tissue studied:  breast cancer
 Relevant cell lines - cell types - tissues:  293 (epithelial), MCF-7 (breast cell)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE GSK3B (human) transfection of constitutively active enzyme, pharmacological inhibitor of upstream enzyme, co-immunoprecipitation, mutation in upstream enzyme recognition motif, transfection of inactive enzyme, transfection of wild-type enzyme
Downstream Regulation
 Effect of modification (function):  intracellular localization


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