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Orthologous residues
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FANCA (human): S1449‑p, FANCA (mouse): Y1433‑p
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Characterization
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Methods used to characterize site in vivo:
immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
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Disease tissue studied:
Fanconi's anaemia
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Relevant cell lines - cell types - tissues:
DK0064, GM01389D, GM6914 (fibroblast), HeLa (cervical)
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Cellular systems studied:
cell lines
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Species studied:
human
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Enzymes shown to modify site in vitro:
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Upstream Regulation
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Potential in vivo enzymes for site:
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Type
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Enzyme
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Evidence
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Notes
|
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KINASE
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ATR (human)
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genetic knockout/knockin of upstream enzyme, phospho-motif antibody, pharmacological inhibitor of upstream enzyme
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Treatments, proteins and their effect on site modification:
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Treatments
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Referenced Treatments
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Manipulated Protein
|
Referenced Protein
|
Effect
|
Notes
|
|
MMC
|
|
|
|
increase
|
|
|
okadaic acid
|
|
|
|
increase
|
|
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wortmannin
|
|
|
|
decrease
|
|
|
SB28078
|
|
|
|
no change compared to control
|
|
|
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Downstream Regulation
|
|
Effect of modification (function):
molecular association, regulation, ubiquitination
|
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Effect of modification (process):
chromatin organization, altered
|
|
Modification regulates interactions with:
|
|
Interacting molecule
|
Interacting domains
|
Effect
|
Consequences (function)
|
Consequences (process)
|
Detection assays
|
|
DNA
|
|
Induces
|
|
|
co-immunoprecipitation
|
|
|
Comments:
level of monoubiquitinated FANCD2 was decreased in FANCA S1449A mutant
|
