Curated Information
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Curated Information Page
PubMed Id: 19060924 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Lodyga M, et al. (2009) XB130, a tissue-specific adaptor protein that couples the RET/PTC oncogenic kinase to PI 3-kinase pathway. Oncogene 28, 937-49 19060924
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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Y54-p - AFAP1L2 (human)
Orthologous residues
AFAP1L2 (human): Y54‑p, AFAP1L2 (mouse): Y54‑p, AFAP1L2 (rat): Y54‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
 Disease tissue studied:  thyroid cancer
 Relevant cell lines - cell types - tissues:  293 (epithelial), TPC-1 (thyroid cell)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Ret (human) co-immunoprecipitation, transfection of wild-type enzyme, transfection of dominant-negative enzyme, pharmacological inhibitor of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ZD6474 decrease
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
PIK3R1 (human) SH2 Induces pull-down assay

S473-p - Akt1 (human)
Orthologous residues
Akt1 (human): S473‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
Ret (human) increase
AFAP1L2 (human) increase siRNA decreases phosphorylation

Y826-p - Ret (human)
Orthologous residues
Ret (human): Y826‑p, Ret iso2 (human): Y826‑p, Ret iso3 (human): Y350‑p, Ret (mouse): Y827‑p, Ret iso2 (mouse): Y827‑p, Ret iso4 (mouse): Y776‑p, Ret (rat): Y827‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

Y1015-p - Ret (human)
Orthologous residues
Ret (human): Y1015‑p, Ret iso2 (human): Y1015‑p, Ret iso3 (human): Y539‑p, Ret (mouse): Y1016‑p, Ret iso2 (mouse): Y1016‑p, Ret iso4 (mouse): , Ret (rat): Y1016‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

Y1029-p - Ret (human)
Orthologous residues
Ret (human): Y1029‑p, Ret iso2 (human): Y1029‑p, Ret iso3 (human): Y553‑p, Ret (mouse): Y1030‑p, Ret iso2 (mouse): Y1030‑p, Ret iso4 (mouse): , Ret (rat): Y1030‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

Y1062-p - Ret (human)
Orthologous residues
Ret (human): Y1062‑p, Ret iso2 (human): Y1062‑p, Ret iso3 (human): Y586‑p, Ret (mouse): Y1063‑p, Ret iso2 (mouse): Y1063‑p, Ret iso4 (mouse): , Ret (rat): Y1063‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human


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