Curated Information
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PubMed Id: 19047061 
Wang C, et al. (2009) Protein Kinase C {theta} (PKC{theta})-dependent Phosphorylation of PDK1 at Ser504 and Ser532 Contributes to Palmitate-induced Insulin Resistance. J Biol Chem 284, 2038-44 19047061
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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T308-p - Akt1 (mouse)
Orthologous residues
Akt1 (human): T308‑p, Akt1 (mouse): T308‑p, Akt1 (rat): T308‑p, Akt1 (fruit fly): T423‑p, Akt1 (cow): T308‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  C2C12 (myoblast), MEF (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse, rat
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PDK1 (mouse) inhibition of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
palmitate insulin inhibit treatment-induced increase
insulin PKCT (mouse) inhibit treatment-induced increase
PDGF increase
palmitate PDGF inhibit treatment-induced increase

S244-p - PDK1 (mouse)
Orthologous residues
PDK1 (human): S241‑p, PDK1 iso4 (human): S114‑p, PDK1 (mouse): S244‑p, PDK1 (rat): S244‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  C2C12 (myoblast), MEF (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse, rat
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
palmitate insulin inhibit treatment-induced increase
insulin PKCT (mouse) inhibit treatment-induced increase
PDGF increase
PDGF palmitate inhibit treatment-induced increase

S504-p - PDK1 (mouse)
Orthologous residues
PDK1 (human): S501‑p, PDK1 iso4 (human): S374‑p, PDK1 (mouse): S504‑p, PDK1 (rat): S504‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  C2C12 (myoblast), MEF (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE PKCT (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PKCT (mouse) genetic knockout/knockin of upstream enzyme, pharmacological inhibitor of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
palmitate increase
SP600125 palmitate inhibit treatment-induced increase
Go 6983 palmitate inhibit treatment-induced increase
Downstream Regulation
 Effect of modification (function):  enzymatic activity, inhibited

S532-p - PDK1 (mouse)
Orthologous residues
PDK1 (human): S529‑p, PDK1 iso4 (human): S402‑p, PDK1 (mouse): S532‑p, PDK1 (rat): S532‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  C2C12 (myoblast), MEF (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE PKCT (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PKCT (mouse) genetic knockout/knockin of upstream enzyme, pharmacological inhibitor of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
palmitate increase
SP600125 palmitate inhibit treatment-induced increase
Go 6983 palmitate inhibit treatment-induced increase
Downstream Regulation
 Effect of modification (function):  enzymatic activity, inhibited


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