Curated Information
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Curated Information Page
PubMed Id: 15916964 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Bhoumik A, et al. (2005) ATM-dependent phosphorylation of ATF2 is required for the DNA damage response. Mol Cell 18, 577-87 15916964
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S490-p - ATF-2 (human) ▲
Orthologous residues
ATF‑2 (human): S490‑p, ATF‑2 (mouse): S472‑p, ATF‑2 (rat): S472‑p
Characterization
 Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody
 Relevant cell lines - cell types - tissues:  1205Lu (melanocyte), 293T (epithelial), AT22IJE-T (fibroblast), GM00637 (fibroblast), GM5849 (fibroblast), IMR-90 (fibroblast), MeWo (melanocyte), NBS-ILB1 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (human) transfection of wild-type enzyme, transfection of inactive enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing radiation increase
neocarzinostatin increase
UV no change compared to control
Downstream Regulation
 Effect of modification (function):  intracellular localization
 Comments:  S472/480 are important for ionizing radiation-induced S phase checkpoint control

S498-p - ATF-2 (human) ▲
Orthologous residues
ATF‑2 (human): S498‑p, ATF‑2 (mouse): S480‑p, ATF‑2 (rat): S480‑p
Characterization
 Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody
 Relevant cell lines - cell types - tissues:  1205Lu (melanocyte), 293T (epithelial), AT22IJE-T (fibroblast), GM00637 (fibroblast), GM5849 (fibroblast), IMR-90 (fibroblast), MeWo (melanocyte), NBS-ILB1 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (human) transfection of wild-type enzyme, transfection of inactive enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing radiation increase
neocarzinostatin increase
UV no change compared to control
Downstream Regulation
 Effect of modification (function):  intracellular localization
 Comments:  S472/480 are important for ionizing radiation-induced S phase checkpoint control

S1981-p - ATM (human) ▲
Orthologous residues
ATM (human): S1981‑p, ATM (mouse): S1987‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody
 Relevant cell lines - cell types - tissues:  GM00637 (fibroblast), NBS-ILB1 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing radiation increase

S317-p - Chk1 (human) ▲
Orthologous residues
Chk1 (human): S317‑p, Chk1 (mouse): S317‑p, Chk1 (rat): S317‑p, Chk1 (chicken): S317‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody
 Relevant cell lines - cell types - tissues:  GM00637 (fibroblast), NBS-ILB1 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing radiation increase

T68-p - Chk2 (human) ▲
Orthologous residues
Chk2 (human): T68‑p, Chk2 iso12 (human): T68‑p, Chk2 (mouse): T77‑p, Chk2 (rat): T76‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody
 Relevant cell lines - cell types - tissues:  GM00637 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing radiation increase


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