Curated Information
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PubMed Id: 14603261 
Wan X, Helman LJ (2003) Levels of PTEN protein modulate Akt phosphorylation on serine 473, but not on threonine 308, in IGF-II-overexpressing rhabdomyosarcomas cells. Oncogene 22, 8205-11 14603261
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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T308-p - Akt1 (human)
Orthologous residues
Akt1 (human): T308‑p, Akt1 (mouse): T308‑p, Akt1 (rat): T308‑p, Akt1 (fruit fly): T423‑p, Akt1 (cow): T308‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Disease tissue studied:  rhabdomyosarcoma
 Relevant cell lines - cell types - tissues:  C2C12 (myoblast), Rh13 (muscle cell), Rh18 (muscle cell), Rh30 (muscle cell), Rh4 (muscle cell), Rh5 (muscle cell)
 Cellular systems studied:  cell lines
 Species studied:  human, mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IGF-2 increase
wortmannin IGF-2 inhibit treatment-induced increase
wortmannin decrease

S473-p - Akt1 (human)
Orthologous residues
Akt1 (human): S473‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Disease tissue studied:  rhabdomyosarcoma
 Relevant cell lines - cell types - tissues:  C2C12 (myoblast), Rh13 (muscle cell), Rh18 (muscle cell), Rh30 (muscle cell), Rh4 (muscle cell), Rh5 (muscle cell)
 Cellular systems studied:  cell lines
 Species studied:  human, mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IGF-2 increase
wortmannin IGF-2 inhibit treatment-induced increase
alphaIR3 IGF-2 inhibit treatment-induced increase
wortmannin decrease


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