Curated Information
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Curated Information Page
PubMed Id: 12591925 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Bauer PM, et al. (2003) Compensatory phosphorylation and protein-protein interactions revealed by loss of function and gain of function mutants of multiple serine phosphorylation sites in endothelial nitric-oxide synthase. J Biol Chem 278, 14841-9 12591925
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S116-p - eNOS (cow) ▲
Orthologous residues
eNOS (human): S114‑p, eNOS (mouse): T113‑p, eNOS (rat): T113‑p, eNOS (rabbit): T120‑p, eNOS (pig): S116‑p, eNOS (cow): S116‑p
Characterization
 Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody
 Relevant cell lines - cell types - tissues:  BAEC (endothelial), COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  cow, monkey
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
VEGF decrease
ATP decrease
Downstream Regulation
 Effect of modification (function):  enzymatic activity, inhibited, molecular association, regulation, phosphorylation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Akt1 (human) Disrupts co-immunoprecipitation, electrophoretic visualization
 Comments:  eNOS S115A mutation increases phosphorylation of S1178 by about 50%

S617-p - eNOS (cow) ▲
Orthologous residues
eNOS (human): S615‑p, eNOS (mouse): S614‑p, eNOS (rat): S614‑p, eNOS (rabbit): S621‑p, eNOS (pig): S617‑p, eNOS (cow): S617‑p
Characterization
 Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody
 Relevant cell lines - cell types - tissues:  BAEC (endothelial), COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  cow, monkey
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
VEGF increase
ATP increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation, phosphorylation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
HSP90A (human) Disrupts co-immunoprecipitation, electrophoretic visualization
Akt1 (human) Disrupts co-immunoprecipitation, electrophoretic visualization
 Comments:  eNOS S616A mutant shows a reduction in phosphorylation of S115 and an increase in phosphorylation of S1178;

S635-p - eNOS (cow) ▲
Orthologous residues
eNOS (human): S633‑p, eNOS (mouse): S632‑p, eNOS (rat): S632‑p, eNOS (rabbit): S639‑p, eNOS (pig): S635‑p, eNOS (cow): S635‑p
Characterization
 Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody
 Relevant cell lines - cell types - tissues:  BAEC (endothelial), COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  cow, monkey
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
VEGF increase
ATP increase
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced, phosphorylation
 Comments:  2-fold higher phosphorylation of S634 in the S1179A eNOS mutant

S1179-p - eNOS (cow) ▲
Orthologous residues
eNOS (human): S1177‑p, eNOS (mouse): S1176‑p, eNOS (rat): S1176‑p, eNOS (rabbit): S1183‑p, eNOS (pig): S1179‑p, eNOS (cow): S1179‑p
Characterization
 Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody
 Relevant cell lines - cell types - tissues:  BAEC (endothelial), COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  cow, monkey
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
VEGF increase
ATP increase
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced, phosphorylation
 Comments:  2-fold higher phosphorylation of S634 in the S1179A eNOS mutant


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