Curated Information
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Curated Information Page
PubMed Id: 14500679 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Zhan Q, et al. (2003) p21-activated kinase 2 in neutrophils can be regulated by phosphorylation at multiple sites and by a variety of protein phosphatases. J Immunol 171, 3785-93 14500679
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S144-p - PAK1 (mouse) ▲
Orthologous residues
PAK1 (human): S144‑p, PAK1 (mouse): S144‑p, PAK1 (rat): S144‑p
Characterization
 Methods used to characterize site in vivo:  electrophoretic mobility shift, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  neutrophil
 Cellular systems studied:  primary cells
 Species studied:  guinea pig
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
fMLP increase

S199-p - PAK1 (mouse) ▲
Orthologous residues
PAK1 (human): S199‑p, PAK1 (mouse): S199‑p, PAK1 (rat): S198‑p
Characterization
 Methods used to characterize site in vivo:  electrophoretic mobility shift, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  neutrophil
 Cellular systems studied:  primary cells
 Species studied:  guinea pig
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
fMLP increase

S204-p - PAK1 (mouse) ▲
Orthologous residues
PAK1 (human): S204‑p, PAK1 (mouse): S204‑p, PAK1 (rat): S203‑p
Characterization
 Methods used to characterize site in vivo:  electrophoretic mobility shift, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  neutrophil
 Cellular systems studied:  primary cells
 Species studied:  guinea pig
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
fMLP increase

T423-p - PAK1 (mouse) ▲
Orthologous residues
PAK1 (human): T423‑p, PAK1 (mouse): T423‑p, PAK1 (rat): T422‑p
Characterization
 Methods used to characterize site in vivo:  electrophoretic mobility shift, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  neutrophil
 Cellular systems studied:  primary cells
 Species studied:  guinea pig
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
fMLP increase

S20-p - PAK2 (mouse) ▲
Orthologous residues
PAK2 (human): S20‑p, PAK2 (mouse): S20‑p, PAK2 (rat): S20‑p, PAK2 (rabbit): S20‑p
Characterization
 Methods used to characterize site in vivo:  electrophoretic mobility shift, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  neutrophil
 Cellular systems studied:  primary cells
 Species studied:  guinea pig

S141-p - PAK2 (mouse) ▲
Orthologous residues
PAK2 (human): S141‑p, PAK2 (mouse): S141‑p, PAK2 (rat): S141‑p, PAK2 (rabbit): S141‑p
Characterization
 Methods used to characterize site in vivo:  electrophoretic mobility shift, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  neutrophil
 Cellular systems studied:  primary cells
 Species studied:  guinea pig

S192-p - PAK2 (mouse) ▲
Orthologous residues
PAK2 (human): S192‑p, PAK2 (mouse): S192‑p, PAK2 (rat): S192‑p, PAK2 (rabbit): S192‑p
Characterization
 Methods used to characterize site in vivo:  electrophoretic mobility shift, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  neutrophil
 Cellular systems studied:  primary cells
 Species studied:  guinea pig
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
fMLP increase

S197-p - PAK2 (mouse) ▲
Orthologous residues
PAK2 (human): S197‑p, PAK2 (mouse): S197‑p, PAK2 (rat): S197‑p, PAK2 (rabbit): S197‑p
Characterization
 Methods used to characterize site in vivo:  electrophoretic mobility shift, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  neutrophil
 Cellular systems studied:  primary cells
 Species studied:  guinea pig
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
fMLP increase

T402-p - PAK2 (mouse) ▲
Orthologous residues
PAK2 (human): T402‑p, PAK2 (mouse): T402‑p, PAK2 (rat): T402‑p, PAK2 (rabbit): T402‑p
Characterization
 Methods used to characterize site in vivo:  electrophoretic mobility shift, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  neutrophil
 Cellular systems studied:  primary cells
 Species studied:  guinea pig
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
PHOSPHATASE PPP2CA (mouse) pharmacological inhibitor of upstream enzyme
PHOSPHATASE PPP2CB (mouse) pharmacological inhibitor of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
fMLP increase
okadaic acid fMLP augment treatment-induced increase
ascomycin fMLP augment treatment-induced increase
fMLP, cyclosporin A fMLP augment treatment-induced increase
FK506 fMLP augment treatment-induced increase

S154-p - PAK3 (mouse) ▲
Orthologous residues
PAK3 (human): S154‑p, PAK3 iso3 (human): S175‑p, PAK3 (mouse): S154‑p, PAK3 (rat): S139‑p
Characterization
 Enzymes shown to modify site in vitro: 
Type Enzyme
PHOSPHATASE PPP2CA (human)
PHOSPHATASE PPP1CA (human)
PHOSPHATASE PPP2CB (human)

T436-p - PAK3 (mouse) ▲
Orthologous residues
PAK3 (human): T436‑p, PAK3 iso3 (human): T457‑p, PAK3 (mouse): T436‑p, PAK3 (rat): T421‑p
Characterization
 Enzymes shown to modify site in vitro: 
Type Enzyme
PHOSPHATASE PPP2CA (human)
PHOSPHATASE PPP1CA (human)


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